Continuity with ganglionic eminence modulates interkinetic nuclear migration in the neocortical pseudostratified ventricular epithelium.
Continuity with ganglionic eminence modulates interkinetic nuclear migration in the neocortical pseudostratified ventricular epithelium.
复制标题
神经节隆起的连续性调节新皮质假复层心室上皮中的运动核迁移。
DOI：
10.1006/exnr.2001.7676
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
CavinessJr,VS
中科院分区：
文献类型：
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作者：
Miyama,S;Takahashi,T;Goto,T;Bhide,PG;CavinessJr,VS
假复层心室上皮细胞（PVE）经历核间迁移，细胞索马与细胞核的位置系统地依赖于细胞周期时相。我们研究了是否在neopallial PVE的运动间核迁移的组织连续性与基底前脑的神经节隆起（GE）在胚胎第13天的小鼠外植体的影响。我们发现，当neopalial壁和GE之间的连续性是完整的，一些neopalial PVE细胞停止interkinetic核迁移后，S期和进入G2期。这些细胞的胞体和细胞核从S期区向外移向室管膜下区和中间区。后S期细胞的向外迁移仅在与GE紧密相邻的大脑壁的外侧区域中观察到，但在内侧区域中没有，并且仅当与GE保持组织连续性时才观察到。我们认为，向外移动PVE细胞种子的次级增殖群体（SPP）和SPP种子细胞的退出发生在G2期。该现象概括了体内新肛PVE细胞的类似迁移行为，并且似乎代表PVE的后S期细胞可获得的两个相反选项之间的“选择”。这种选择似乎是由依赖于与通用电气公司的连续性的机制强加的。我们的结论是，GE，和/或其他相邻的基底前脑结构，调制interkinetic核迁移在neopallial PVE。
Cells of the pseudostratified ventricular epithelium (PVE) undergo interkinetic nuclear migration whereby position of cell soma with nucleus is systematically dependent upon cell cycle phase. We examined if the interkinetic nuclear migration in the neopallial PVE is influenced by tissue continuity with the ganglionic eminence (GE) of the basal forebrain in explants from embryonic day 13 mice. We found that when continuity between the neopallial wall and the GE is intact, some neopallial PVE cells discontinue interkinetic nuclear migration following S-phase and upon entry into G2-phase. The somata and nuclei of those cells shift outward from the S-phase zone toward the subventricular and the intermediate zones. The outward migration of post-S-phase cells is observed only in the lateral region of the cerebral wall, which is closely adjacent to the GE, but not in the medial region, and only when tissue continuity with GE is maintained. We suggest that the outward moving PVE cells seed the secondary proliferative population (SPP) and that exit of the SPP seeding cells occurs in G2-phase. The phenomenon recapitulates similar migratory behavior of neopallial PVE cells in vivo and appears to represent a “choice” between two opposing options available to post-S-phase cells of the PVE. The choice appears to be imposed by mechanisms dependent upon continuity with the GE. We conclude that GE, and/or other adjacent basal forebrain structures, modulates interkinetic nuclear migration in the neopallial PVE.