MEMBRANE-POTENTIAL CHANGES ASSOCIATED WITH DIFFERENTIATION OF ENTEROCYTES IN THE RAT INTESTINAL VILLI IN CULTURE
MEMBRANE-POTENTIAL CHANGES ASSOCIATED WITH DIFFERENTIATION OF ENTEROCYTES IN THE RAT INTESTINAL VILLI IN CULTURE
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DOI：
10.1016/0012-1606(82)90348-7
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
OKADA, Y
中科院分区：
文献类型：
--
作者：
TSUCHIYA, W;OKADA, Y
从新生大鼠分离的小肠碎片绒毛保持在培养长达4周的时间。在培养中，漂浮的球形绒毛和粘附绒毛被区分。细胞内记录从两种类型的绒毛。膜电位最高（约0.0001）。-70 mV），并且最低（约70 mV）。-18 mV），显示出根据细胞位置的连续渐变。培养的成熟肠上皮细胞的膜电位是由于产电Na+泵的贡献，以及钙激活的K+电导。这两个组件的温度和代谢能量依赖。Ca 2+转运机制和产电Na+泵可能参与了肠上皮细胞的分化或成熟过程。
Fragmented villi of the small intestine isolated from newborn rats were maintained in culture for periods of up to 4 wk. In culture, floating globular villi and adherent villi were distinguished. Intracellular recordings were made from both types of villi. The membrane potential was highest (.apprx. -70 mV) in the cells at the tip of the villus and was lowest (.apprx. -18 mV) in the cells near the crypt region, showing a continuous gradation according to the cell location. The membrane potential in cultured mature enterocytes was due to the contribution of the electrogenic Na+ pump, as well as to the Ca2+-activated K+ conductance. Both the components were temperature- and metabolic energy-dependent. The Ca2+ transport mechanism and the electrogenic Na+ pump are probably involved in the process of cell differentiation or maturation of the intestinal epithelia.