Root exudation of coumarins from soil-grown Arabidopsis thaliana in response to iron deficiency
Root exudation of coumarins from soil-grown Arabidopsis thaliana in response to iron deficiency
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DOI：
10.1016/j.rhisph.2020.100296
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发表时间：
2021-03-01
期刊：
影响因子：
3.7
通讯作者：
Puschenreiter, Markus
中科院分区：
文献类型：
--
作者：
Rosenkranz, Theresa;Oburger, Eva;Puschenreiter, Markus
在碱性土壤中，铁（Fe）的有效性是有限的，植物开发了不同的策略来克服这一限制。拟南芥遵循基于还原的策略，其中Fe在植物吸收之前在根表面被还原。最近的研究强调了苯丙烷衍生的香豆素在A. thaliana.在这项研究中，我们种植了A. thaliana野生型Col-O和f6'h1突变体缺陷的苯丙烷衍生的香豆素合成，在六个对比的土壤和确定香豆素分泌速率和根际的生化变化。在天然碱性土壤上，f6'h1突变体不能生长，只能通过定期补铁维持生长。从土壤生长的野生型植物在清洗根后水培取样根分泌物。东莨菪内酯是A.生长在不同土壤上的拟南芥。东莨菪碱的渗出率增加，降低土壤中的铁的生物利用度，与酸性土壤中的植物表现出类似的渗出率在碱性土壤中测量的速率除外。这是首次报道从A.生长在一系列具有不同铁有效性的天然土壤上的拟南芥。
In alkaline soils, iron (Fe) availability is limited and plants developed different strategies to overcome this constraint. Arabidopsis thaliana follows a reduction-based strategy, in which Fe is reduced at the root surface before plant uptake. Recent studies highlighted the role of phenylpropanoid-derived coumarins in the mobilisation and uptake of Fe in A. thaliana. In this study, we grew the A. thaliana wild-type Col-O and the f6'h1 mutant defective in phenylpropanoid-derived coumarin synthesis, on six contrasting soils and determined coumarin exudation rates and biochemical changes in the rhizosphere. On the natural alkaline soils, the f6'h1 mutant was not able to grow and growth could only be sustained with regular Fe supply. Root exudates were sampled hydroponically from soil-grown wild-type plants after washing roots. Scopoletin was the predominant coumarin found in the exudates of A. thaliana grown on the different soils. Exudation rates of scopoletin increased with decreasing bioavailability of Fe in soil, with the exception of the acidic soil where plants showed exudation rates similar to the rates measured in alkaline soils. This is the first study reporting exudation of coumarins from A. thaliana grown on a range of natural soils with varying Fe availability.