Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway
Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway
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DOI：
10.1016/j.febslet.2009.05.024
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发表时间：
2009-06-18
期刊：
影响因子：
3.5
通讯作者：
Morimoto, Satoshi
中科院分区：
文献类型：
--
作者：
Taura, Futoshi;Tanaka, Shinji;Morimoto, Satoshi
大麻素的烷基间苯二酚部分衍生自橄榄酚酸（奥拉），一种聚酮化合物代谢物。然而，负责奥拉生物合成的聚酮合酶（PKS）尚未被鉴定。在本研究中，一个新的PKS，橄榄醇合酶（OLS）的cDNA编码，从大麻克隆。重组OLS不产生奥拉，但合成橄榄醇，脱羧形式的奥拉，作为主要的反应产物。有趣的是，还证实了从花和快速扩展的叶中提取的粗酶提取物，C. sativa也表现出产生橄榄醇的活性，表明天然OLS在这些组织中功能性表达。根据OLS的催化性质和表达产物，探讨了OLS参与奥拉生物合成的可能性。乐(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Alkylresorcinol moieties of cannabinoids are derived from olivetolic acid (OLA), a polyketide metabolite. However, the polyketide synthase (PKS) responsible for OLA biosynthesis has not been identified. In the present study, a cDNA encoding a novel PKS, olivetol synthase (OLS), was cloned from Cannabis sativa. Recombinant OLS did not produce OLA, but synthesized olivetol, the decarboxylated form of OLA, as the major reaction product. Interestingly, it was also confirmed that the crude enzyme extracts from flowers and rapidly expanding leaves, the cannabinoid-producing tissues of C. sativa, also exhibited olivetol-producing activity, suggesting that the native OLS is functionally expressed in these tissues. The possibility that OLS could be involved in OLA biosynthesis was discussed based on its catalytic properties and expression pro. le. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.