The novel long noncoding RNA AC138128.1 may be a predictive biomarker in gastric cancer
The novel long noncoding RNA AC138128.1 may be a predictive biomarker in gastric cancer
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新型长非编码RNA AC138128.1可能是胃癌的预测生物标志物
DOI：
10.1007/s12032-014-0262-7
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发表时间：
2014-11-01
期刊：
影响因子：
3.4
通讯作者：
Wang, Zhenning
中科院分区：
文献类型：
--
作者：
Chen, Xiaowan;Sun, Jingxu;Wang, Zhenning
众所周知，长链非编码RNA（lncRNA）的差异表达在肿瘤发生中起着关键作用。然而，lncRNA在胃癌（GC）诊断中的意义仍不清楚。本研究的目的是确定一种新的lncRNA AC138128.1在GC中的表达水平及其临床相关性。应用实时荧光定量逆转录聚合酶链反应（RT-PCR）检测94对胃癌组织及癌旁正常组织（NAT）中AC138128.1的表达。分析胃癌组织中AC138128.1表达水平与临床病理特征之间的潜在相关性。最后，构建受试者工作特征（ROC）曲线。结果表明，胃癌组织中AC138128.1的表达水平明显低于正常胃黏膜组织（p < 0.001）。GC细胞系AGS BGC-823、HG 27和SGC-7901中的AC 138128.1水平低于人正常胃上皮细胞系GES-1中的水平。ROC曲线下面积为0.688。本研究表明lncRNA AC 138128.1可能是预测GC的一种新的生物标志物。
It is well known that the differential expression of long noncoding RNA (lncRNA) plays critical roles in carcinogenesis. However, the significance of lncRNA in the diagnosis of gastric cancer (GC) remains largely unknown. The goal of this study was to determine the expression level of AC138128.1, a novel lncRNA, in GC and its clinical association. The expression of AC138128.1 in 94 pairs of tumors and normal adjacent tissues (NAT) from patients with GC was detected by quantitative real-time reverse transcription polymerase chain reaction. The potential association between AC138128.1 expression level in GC tissue and clinicopathological features was analyzed. Finally, a receiver operating characteristic (ROC) curve was constructed. The results showed that the expression level of AC138128.1 in GC was significantly decreased compared to that in NAT (p < 0.001). Levels of AC138128.1 in the GC cell lines, AGS BGC-823, HG27, and SGC-7901 were lower than those in the human normal gastric epithelial cell line GES-1. Moreover, the area under the ROC curve was 0.688. This study demonstrates that lncRNA AC138128.1 might be a novel biomarker for predicting GC.