Real-time temperature correction for magnetoresistive biosensors integrated with temperature modulator.
Real-time temperature correction for magnetoresistive biosensors integrated with temperature modulator.
复制标题
与温度调制器集成的磁阻生物传感器的实时温度校正。
DOI：
10.1016/j.biosx.2023.100356
复制
发表时间：
2023
影响因子：
--
通讯作者：
Lee,Jung-Rok
中科院分区：
文献类型：
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作者：
Kim,Songeun;Wang,ShanX;Lee,Jung-Rok
基于磁阻的生物传感器利用不同磁场下电阻的变化来测量与磁标签有关的生物分子或事件。然而，电阻随温度而波动。为了从感兴趣的信号中解耦不需要的温度相关信号，已经提出了各种方法来校正基于磁阻的生物传感器的信号。然而，仍然需要一种能够即时校正阵列中所有传感器信号的温度校正方法，因为需要在中心实验室或护理点设置中使用一组传感器同时检测多种生物标志物。在这里，我们报告了一种巨磁阻生物传感器系统，该系统可以使用通过集成温度调制器产生的温度扫描获得的温度校正系数对单个传感器进行实时温度校正。使用单个温度校正系数的算法明显优于使用平均温度校正系数的算法。此外，温度调节并不能完全消除与温度相关的信号。为了证明该方法可用于涉及较大温度变化的生物医学应用，使用温度校正方法进行了结合动力学实验和熔化曲线分析。该方法成功地去除了所有与温度相关的伪影，从而产生了更精确的动力学参数和DNA杂交体的熔化温度。
Magnetoresistance-based biosensors utilize changes in electrical resistance upon varying magnetic fields to measure biological molecules or events involved with magnetic tags. However, electrical resistance fluctuates with temperature. To decouple unwanted temperature-dependent signals from the signal of interest, various methods have been proposed to correct signals from magnetoresistance-based biosensors. Yet, there is still a need for a temperature correction method capable of instantaneously correcting signals from all sensors in an array, as multiple biomarkers need to be detected simultaneously with a group of sensors in a central laboratory or point-of-care setting. Here we report a giant magnetoresistive biosensor system that enables real-time temperature correction for individual sensors using temperature correction coefficients obtained through a temperature sweep generated by an integrated temperature modulator. The algorithm with individual temperature correction coefficients obviously outperformed that using the average temperature correction coefficient. Further, temperature regulation did not eliminate temperature-dependent signals completely. To demonstrate that the method can be used in biomedical applications where large temperature variations are involved, binding kinetics experiments and melting curve analysis were conducted with the temperature correction method. The method successfully removed all temperature-dependent artifacts and thus produced more precise kinetic parameters and melting temperatures of DNA hybrids.