Drosophila wing imaginal discs respond to mechanical injury via slow InsP3R-mediated intercellular calcium waves
Drosophila wing imaginal discs respond to mechanical injury via slow InsP3R-mediated intercellular calcium waves
复制标题
果蝇翼成虫盘通过缓慢的 InsP3R 介导的细胞间钙波对机械损伤做出反应
作者：
Simon Restrepo;K. Basler
钙信号是一种高度通用的细胞通讯系统，它调节基本功能，如细胞收缩，动物发育的基本步骤，如受精，以及更高级别的过程，如记忆。我们通过体内外成像和遗传分析相结合的方法，探讨了果蝇翅膀成像盘中钙信号的功能。在这里，我们发现，当在体内或体外培养时，翅盘显示出缓慢的、远程的细胞间钙波(ICW)。这些慢的想象盘细胞间钙波(SIDICs)由肌醇-3-磷酸受体、内质网(ER)钙泵SERCA和关键缝隙连接成分Inx2介导。SIDIC形成和繁殖所需的基因被敲除，对机械损伤后翼盘的恢复产生负面影响。我们的结果揭示了ICW在翼盘动态平衡中的作用，并强调了翼盘作为钙信号研究的模型的实用性。目前尚不清楚钙信号在果蝇翼盘中扮演什么角色。在此，作者发现在机械应力下，在体内和体外，由肌醇-3-磷酸受体、钙泵SERCA和缝隙连接成分Inx2介导的缓慢、长程的细胞间钙波被启动。
Calcium signalling is a highly versatile cellular communication system that modulates basic functions such as cell contractility, essential steps of animal development such as fertilization and higher-order processes such as memory. We probed the function of calcium signalling in Drosophila wing imaginal discs through a combination of ex vivo and in vivo imaging and genetic analysis. Here we discover that wing discs display slow, long-range intercellular calcium waves (ICWs) when mechanically stressed in vivo or cultured ex vivo. These slow imaginal disc intercellular calcium waves (SIDICs) are mediated by the inositol-3-phosphate receptor, the endoplasmic reticulum (ER) calcium pump SERCA and the key gap junction component Inx2. The knockdown of genes required for SIDIC formation and propagation negatively affects wing disc recovery after mechanical injury. Our results reveal a role for ICWs in wing disc homoeostasis and highlight the utility of the wing disc as a model for calcium signalling studies. It is unclear what role calcium signalling plays in the Drosophila wing disc. Here, the authors show that on mechanical stress, slow, long-range intercellular calcium waves are initiated in vivo and ex vivo, mediated by the inositol-3-phosphate receptor, the calcium pump SERCA and gap junction component Inx2.
DOI：
10.1042/bj20131429
发表时间：
2014-03-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Mustafi SM;Brecher M;Zhang J;Li H;Lemaster DM;Hernández G
通讯作者：
Hernández G
影响因子：
2.7
作者：
Ferrari, MB;Spitzer, NC
通讯作者：
Spitzer, NC
磷脂酶 C 活性而非兰尼碱受体在细胞间钙波的启动和传播中发挥作用。
影响因子：
4
作者：
Hansen,M;Boitano,S;Dirksen,ER;Sanderson,MJ
通讯作者：
Sanderson,MJ