Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
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DOI：
10.1038/nmeth.1488
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发表时间：
2010-09-01
期刊：
影响因子：
48
通讯作者：
Nagai, Takeharu
中科院分区：
文献类型：
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作者：
Horikawa, Kazuki;Yamada, Yoshiyuki;Nagai, Takeharu
我们报告了超灵敏的Ca 2+指示剂，黄色cameleon纳米（YC纳米），通过工程化的Ca 2+传感结构域的遗传编码的Ca 2+指示剂，YC 2.60或YC 3.60开发。它们的高Ca 2+亲和力（Kd = 15-140 nM）和大信号变化（1，450%）使得即使在100，000个细胞网络中也能够检测与细胞间信号动力学和神经元活性相关的微妙Ca 2+瞬变。这些指标将是有用的研究信息处理的生活多细胞网络。
We report ultrasensitive Ca2+ indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca2+-sensing domain of a genetically encoded Ca2+ indicator, YC2.60 or YC3.60. Their high Ca2+ affinities (K-d = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca2+ transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.