Metabolomic analysis of the response of growing pigs to dietary l-arginine supplementation
Metabolomic analysis of the response of growing pigs to dietary l-arginine supplementation
复制标题
生长猪对日粮补充 L-精氨酸反应的代谢组学分析
DOI：
10.1007/s00726-008-0192-9
复制
发表时间：
2009-05-01
期刊：
影响因子：
3.5
通讯作者：
Wu, Yongning
中科院分区：
文献类型：
--
作者：
He, Qinghua;Kong, Xiangfeng;Wu, Yongning
精氨酸在调节营养物质代谢中起着重要作用，但其潜在机制在很大程度上尚不清楚。本研究旨在利用核磁共振氢谱技术研究日粮中添加精氨酸对生长猪血清代谢物组的影响。选用16头120日龄（48 ± 1 kg）的猪，随机分为两组，分别在玉米和豆粕基础日粮中添加0%和1.0% L-精氨酸。在46天的治疗期后收集血清。饮食中补充精氨酸可减少脂肪沉积，增加体内蛋白质积累。主成分分析表明，血清中的低密度脂蛋白，极低密度脂蛋白，尿素浓度较低，但肌酐，三羧酸循环代谢产物，鸟氨酸，赖氨酸和酪氨酸浓度较高，在补充精氨酸的猪比对照组。此外，精氨酸治疗影响含氮和脂质信号分子（甘油磷酸胆碱和肌醇）和肠道细菌代谢物（甲酸盐，乙醇，甲胺，二甲胺，乙酸盐和丙酸盐）的血清浓度。这些新的发现表明，日粮中补充精氨酸改变了脂肪和氨基酸在整个身体中的催化作用，增强了骨骼肌中的蛋白质合成，并调节了生长猪的肠道微生物代谢。
Arginine plays an important role regulating nutrient metabolism, but the underlying mechanisms are largely unknown. This study was conducted to determine the effect of dietary arginine supplementation on the metabolome in serum of growing pigs using 1H nuclear magnetic resonance spectroscopy. Sixteen 120-day-old pigs (48 ± 1 kg) were randomly assigned to one of two groups, representing supplementation with 0 or 1.0% l-arginine to corn- and soybean meal-based diets. Serum was collected after a 46-day period of treatment. Dietary arginine supplementation decreased fat deposition and increased protein accretion in the body. Principal component analysis showed that serum concentrations of low density lipoprotein, very low density lipoprotein, and urea were lower, but concentrations of creatinine, tricarboxylic acid cycle metabolites, ornithine, lysine and tyrosine were greater in arginine-supplemented than in control pigs. Additionally, the arginine treatment affected serum concentrations of nitrogenous and lipid signaling molecules (glycerophosphorylcholine and myo-inositol) and intestinal bacterial metabolites (formate, ethanol, methylamine, dimethylamine, acetate, and propionate). These novel findings suggest that dietary arginine supplementation alters the catabolism of fat and amino acids in the whole body, enhances protein synthesis in skeletal muscle, and modulates intestinal microbial metabolism in growing pigs.