Transcript and metabolite alterations increase ganoderic acid content in Ganoderma lucidum using acetic acid as an inducer
Transcript and metabolite alterations increase ganoderic acid content in Ganoderma lucidum using acetic acid as an inducer
复制标题
DOI：
10.1007/s10529-014-1636-9
复制
发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Zhao, Ming-Wen
中科院分区：
文献类型：
--
作者：
Ren, Ang;Li, Xiong-Biao;Zhao, Ming-Wen
5-8 mM的乙酸增加灵芝酸（GA）在灵芝中的积累。经响应面法优化后，赤霉素含量达到5.5/100 mg干重，比对照提高了105%。GA生物合成的中间代谢产物羊毛甾醇和角鲨烯也分别增加到47和15.8微克/克干重，响应于乙酸。乙酸显著诱导GA生物合成途径中sqs、lano、hmgs和cyp 51的转录水平。从10个候选同源acs基因中筛选出一个乙酸非调控的乙酰辅酶A合酶基因。结果表明，乙酸改变了与乙酸同化相关的基因的表达，并增加了GA的生物合成和羊毛甾醇，角鲨烯和GA-α的代谢水平，从而导致GA积累。
Acetic acid at 5-8 mM increased ganoderic acid (GA) accumulation in Ganoderma lucidum. After optimization by the response surface methodology, the GA content reached 5.5/100 mg dry weight, an increase of 105 % compared with the control. The intermediate metabolites of GA biosynthesis, lanosterol and squalene also increased to 47 and 15.8 mu g/g dry weight, respectively, in response to acetic acid. Acetic acid significantly induced transcription levels of sqs, lano, hmgs and cyp51 in the GA biosynthesis pathway. An acetic acid-unregulated acetyl coenzyme A synthase (acs) gene was selected from ten candidate homologous acs genes. The results indicate that acetic acid alters the expression of genes related to acetic acid assimilation and increases GA biosynthesis and the metabolic levels of lanosterol, squalene and GA-a, thereby resulting in GA accumulation.