CYCLIC ESTRADIOL REPLACEMENT INCREASES THE SATIETY EFFECT OF CCK-8 IN OVARIECTOMIZED RATS
CYCLIC ESTRADIOL REPLACEMENT INCREASES THE SATIETY EFFECT OF CCK-8 IN OVARIECTOMIZED RATS
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DOI：
10.1016/0031-9384(94)90196-1
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发表时间：
1994-08-01
影响因子：
2.9
通讯作者：
SMITH, GP
中科院分区：
文献类型：
--
作者：
GEARY, N;TRACE, D;SMITH, GP
测定周期性卵巢激素替代疗法对外源性CCK-8饱腹感的影响，探讨介导雌性大鼠发情前进食量减少的机制。每周一次，给禁食的卵巢切除大鼠腹腔注射0.5-4μg/kg CCK-8，并在每天两次皮下注射2.5或10μg苯甲酸雌二醇或媒介物的第二次后52小时以及在500mg黄体酮或媒介物后4小时提供0.4-0.8M蔗糖。在三项测试中，雌二醇均显着增加了 CCK-8 对蔗糖摄入的抑制作用。相比之下，单独使用黄体酮或与雌二醇联合使用并不能持续影响 CCK-8 的饱腹感。雌二醇和CCK-8的相互作用在4μg/kg CCK-8剂量时最明显。这种相互作用发生在昼间测试和暗开始测试期间，其中雌二醇不会降低基线蔗糖摄入量。这些结果表明，在卵巢切除大鼠中进行雌二醇替代的循环方案足以增强外源性CCK-8的饱腹感，并且同时黄体酮治疗不会影响这种效果。摄入食物增强小肠释放的CCK的饱腹感可能是发情周期雌激素浓度高期间食物摄入量减少的机制之一。
The influence of cyclic ovarian hormone replacement therapy on the satiety effect of exogenous CCK-8 was determined to investigate the mechanism mediating the preestrous decrease in meal size in female rats. Once weekly, food-deprived ovariectomized rats were IP injected with 0.5-4 mu g/kg CCK-8 and offered 0.4-0.8 M sucrose 52 h after the second of two daily SC injections of 2.5 or 10 mu g estradiol benzoate or vehicle and 4 h after 500 mg progesterone or vehicle. In each of three tests, estradiol significantly increased CCK-8's inhibitory effect on sucrose intake. In contrast, progesterone alone or in combination with estradiol did not consistently influence the satiating potency of CCK-8. The interaction of estradiol and CCK-8 was dearest for the dose of 4 mu g/kg CCK-8. The interaction occurred during diurnal tests and during dark-onset tests in which estradiol did not decrease baseline sucrose intake. These results demonstrate that a cyclic regimen of estradiol replacement in ovariectomized rats is sufficient to enhance the satiating effect of exogenous CCK-8 and that simultaneous progesterone treatment does not influence this effect. Potentiation of the satiating effect of CCK released from the small intestine by ingested food may be one of the mechanisms by which food intake decreases during the period of high estrogen concentration in the estrus cycle.