DIFFERENTIATION OF GRANULE CELL DENDRITES IN THE DENTATE GYRUS OF THE RHESUS-MONKEY - A QUANTITATIVE GOLGI-STUDY
DIFFERENTIATION OF GRANULE CELL DENDRITES IN THE DENTATE GYRUS OF THE RHESUS-MONKEY - A QUANTITATIVE GOLGI-STUDY
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DOI：
10.1002/cne.902140210
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
RAKIC, P
中科院分区：
文献类型：
--
作者：
DUFFY, CJ;RAKIC, P
采用快速高尔基法研究了发育中的恒河猴海马齿状回颗粒细胞树突的分化情况。在胚胎第58天、第95天、第120天、第153天、第165天、出生后第3天、第20天、第60天、第150天、第365天和成虫的树突总长度、树突棘总数和树突轴近、中、远三分之一的树突棘密度。在所有年龄的研究中，颗粒细胞表现出不同程度的成熟，分化程度较高的细胞位于颗粒层的表层，而较不成熟的细胞则逐渐位于较深的位置，从而符合它们形成的由外到内的时空梯度。定量分析表明，该灵长类动物海马颗粒细胞主要在妊娠后半期分化，所有测量参数在出生时达到成熟值。分析还揭示了旺盛的出生后发育的短暂阶段，包括过度的树突分支，树突长度的区域变化，树突棘的过量产生和分子层内棘的重新分布。在大约一岁中期达到峰值后，这些参数下降，并在成年猴子中回落到新生儿水平。
The differentiation of granule cell dendrites in the dentate gyrus of the hippocampal region was studied in a series of developing fetal and postnatal rhesus monkeys whose brains were processed by the rapid Golgi method. The total combined lengths of dendrites, the total number of dendritic spines and their density on the proximal, middle and distal thirds of the dendritic shafts were determined at embryonic days 58, 95, 120, 153, term (165), postnatal days 3, 20, 60, 150, 365 and adults. At all ages examined, granule cells exhibited various levels of maturation with the more differentiated cells being situated in the superficial strata of the granular layer and the less mature cells lying in progressively deeper positions, thus conforming to the outside-to-inside spatiotemporal gradient of their genesis. Quantitative analysis shows that, in this primate, hippocampal granule cells differentiate mainly in the 2nd half of gestation with all measured parameters attaining mature values by the time of birth. The analysis also reveals a transient phase of exuberant postnatal development which involves excessive dendritic branching, regional changes in dendritic length, overproduction of dendritic spines and redistribution of spines within the molecular layer. After reaching peak values around the middle of the 1st yr of life, these parameters decrease and in adult monkeys fall back to the neonatal level.