Comprehensive transcriptomic analysis in response to abscisic acid in Salvia miltiorrhiza
Comprehensive transcriptomic analysis in response to abscisic acid in Salvia miltiorrhiza
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DOI：
10.1007/s11240-021-02135-x
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发表时间：
2021-07-08
影响因子：
3
通讯作者：
Kai, Guoyin
中科院分区：
文献类型：
--
作者：
Shi, Min;Hua, Qiang;Kai, Guoyin
aba处理丹参关键信息转录组学分析。酚酸和丹参酮是中药丹参中积累的具有多种药理作用的重要生物活性成分。脱落酸（ABA）能有效提高丹参中这两种化合物的含量，但其调控机制尚不清楚。对经ABA处理的丹参毛状根进行了深度转录组分析。共从28.1亿个clean reads中获得42710个平均长度为1157 bp的unigenes，其中27641个unigenes（64.72%）被成功注释。编码PAL、TAT、C4H、RAS、CYP98A14、GGPPS等关键酶的基因表达量显著高于对照。筛选出与RAS和CYP98A14在酚酸生物合成中表达相似的候选漆酶，以及可能参与丹参酮生物合成基因的P450单基因。此外，转录因子（TF）谱显示，鉴定出35个家族的1,250个TF，其中MYB、AP2/ERF、bHLH和bZIP最为丰富。本研究提供了受ABA影响的全面的转录表达谱，有助于了解ABA的生物合成和调控机制。
Key message Transcriptomic analysis of ABA-treated Salvia miltiorrhiza.Phenolic acids and tanshinones are important bioactive components with multiple pharmacological properties accumulated in traditional Chinese medicinal plant Salvia miltiorrhiza. Abscisic acid (ABA) is effective to enhance the content of the two compounds in S. miltiorrhiza, while the regulation mechanism remains unknown. Deep transcriptome profile of S. miltiorrhiza hairy roots treated with ABA was conducted. Totally, 42,710 unigenes with the average length of 1,157 bp were obtained from de novo assembly from about 2.81 billion produced clean reads, of which 27,641 unigenes (64.72%) were successfully annotated. Unigenes encoding key enzymes such as PAL, TAT, C4H, RAS, CYP98A14, GGPPS expressed significantly higher than that in control. Candidate laccases showing similar expression with RAS and CYP98A14 in phenolic acid biosynthesis and potential P450 unigenes involved in tanshinone biosynthesis genes were screened out. Furthermore, transcription factor (TF) profiles revealed that 1,250 TFs representing 35 families were identified, of which MYB, AP2/ERF, bHLH and bZIP were most abundant. The present study provided a comprehensive transcriptional expression profile affected by ABA, which will help underly the biosynthesis and regulation mechanism.