FAS-mediated circRNA-miRNA-mRNA Crosstalk Network Regulates Immune Cell Infiltration in Cerebral Infarction
FAS-mediated circRNA-miRNA-mRNA Crosstalk Network Regulates Immune Cell Infiltration in Cerebral Infarction
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DOI：
10.1007/s12031-023-02100-7
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发表时间：
2023-01-19
影响因子：
3.1
通讯作者：
Ren, Bo
中科院分区：
文献类型：
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作者：
Ying, Ke;Chen, Juan;Ren, Bo
有关脑梗塞(CI)中环状RNAs(CircRNAs)、微RNAs(miRNAs/miRs)和信使RNAs(MRNAs)之间相互作用的新数据正在积累。这项研究旨在阐明基于GEO数据库的CircRNA-miRNA-mRNA串扰网络在脑梗塞免疫细胞渗透中的识别。差异分析表明，从GEO数据库中检索到在CI样本中差异表达的1696个CircRNAs、1989个miRNAs和5550个mRNAs。GO和KEGG功能丰富分析表明，差异表达的mRNAs主要与CI的常见危险因素有关，如免疫和炎症反应。其次，预测了CircRNA-miRNA和miRNA-mRNA对，并利用Cytoscape软件构建了CircRNA-miRNA-mRNA网络。通过在线数据库共获得436对CircRNA-miRNA，其中2033对miRNA-mRNA用于构建CircRNA-miRNA-mRNA串扰网络。在CERNA网络的基础上构建了蛋白质-蛋白质相互作用(PPI)网络，并在GSE9877数据集中进行了关键基因的识别。Fas基因被认为是脑梗塞的关键基因。构建的Fas介导的CircRNA-miRNA串扰网络包括5个上调的CircRNAs(hsa_circ_0075341、hsa_circ_0049637、hsa_circ_0001085、hsa_circ_0004808和hsa_circ_0092337)和5个下调的miRNAs(hsa-miR-92a-2-5p、hsa-miR-1245b-3p、hsa-miR-592、hsa-miR-224-5p和hsa-miR-30e-3p)。此外，CiberSort算法表明，Fas与脑梗塞患者的免疫细胞浸润有关。综上所述，本研究揭示了以Fas为中心的CircRNA-miRNA-mRNA串扰网络在调节CI免疫细胞渗透中的作用，这可能是CI预防的一个可行的靶点。
New data are accumulating on the involvement of interaction among circular RNAs (circRNAs), microRNAs (miRNAs/miRs), and messenger RNAs (mRNAs) in cerebral infarction (CI). This study aims to illustrate the GEO database-based identification of a circRNA-miRNA-mRNA crosstalk network underlying immune cell infiltration in CI. The differential analysis suggested that 1696 circRNAs, 1989 miRNAs, and 5550 mRNAs that were differentially expressed in CI samples were retrieved from GEO database. GO and KEGG functional enrichment analyses showed that the differentially expressed mRNAs were mainly associated with common risk factors of CI, such as immune and inflammatory response. Next, the circRNA-miRNA pairs and miRNA-mRNA pairs were predicted, and the circRNA-miRNA-mRNA network was constructed by Cytoscape software. Totally, 436 circRNA-miRNA pairs were obtained through the online database, and 2033 miRNA-mRNA pairs were used to construct the circRNA-miRNA-mRNA crosstalk network. A protein-protein interaction (PPI) network was constructed on the basis of the ceRNA network, followed by key gene identification in the GSE9877 dataset. FAS was identified as the key gene in CI. The constructed FAS-mediated circRNA-miRNA-mRNA crosstalk network included five upregulated circRNAs (hsa_circ_0075341, hsa_circ_0049637, hsa_circ_0001085, hsa_circ_0004808 and hsa_circ_0092337) and five downregulated miRNAs (hsa-miR-92a-2-5p, hsa-miR-1245b-3p, hsa-miR-592, hsa-miR-224-5p, and hsa-miR-30e-3p). Furthermore, the CIBERSORT algorithm indicated that FAS was associated with immune cell infiltration in CI. In conclusion, this study revealed a role for FAS-centered circRNA-miRNA-mRNA crosstalk network in regulating immune cell infiltration of CI, which may be a viable target for CI prevention.