Effect of cold preservation on pulmonary arterial smooth muscle cells.
Effect of cold preservation on pulmonary arterial smooth muscle cells.
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冷藏对肺动脉平滑肌细胞的影响。
DOI：
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发表时间：
1996
影响因子：
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通讯作者：
S. Haworth
中科院分区：
文献类型：
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作者：
S. Hall;S. Haworth
采用EuroCollins液（EC）、威斯康星州大学液（UW）、马歇尔液（MS）和组织培养生长液（GM）对猪肺动脉平滑肌（SM）细胞在冷却和复温过程中的细胞骨架和收缩功能进行了评价。功能研究包括动脉环中前列腺素F2 a（PGF 2a）的被动扩张和收缩，以及冷却-复温培养的SM细胞对硅胶膜的破坏。在冷却的动脉环的肌动蛋白亮度的免疫荧光测量。用SM α-肌动蛋白、SM肌球蛋白和微管蛋白的抗体对培养的SM单层进行染色。在冷却，所有的解决方案导致增加动脉扩张性，而EC和MS减少细胞肿胀。使用所有的解决方案，肌动蛋白电缆变薄，肌球蛋白丝解离，微管解聚。在复温过程中，所有动脉环对施加张力的阻力增加。GM、艾德和MS保存后，对PGF 2a的收缩增加。皱纹增加，肌动蛋白-肌球蛋白电缆缩短后，GM和EC; UW后，皱纹减少，肌动蛋白-肌球蛋白电缆变薄。因此，保存液的类型影响保存期间和复温后的收缩性。在UW中冷却的细胞中不存在自发收缩可能是有利的。
The efficacy of preservation fluids on the cytoskeleton and contractile function of porcine pulmonary arterial smooth muscle (SM) cells during cooling and rewarming was evaluated, using EuroCollins solution (EC), University of Wisconsin solution (UW), Marshall's solution (MS), and tissue culture growth medium (GM). Functional studies included passive distensibility and contraction to prostaglandin F2a (PGF2a) in arterial rings and wrinkling of silicone membranes by cooled-rewarmed cultured SM cells. Immunofluorescence measurements were made of actin brightness in cooled arterial rings. Cultured SM monolayers were stained with antibodies to SM alpha-actin, SM myosin, and tubulin. In cooling, all solutions resulted in increased arterial distensibility, whereas EC and MS reduced cell wrinkling. With the use of all solutions, actin cables thinned, myosin filaments dissociated, and microtubules depolymerized. During rewarming, resistance to imposed tension increased in all arterial rings. After GM,ED, and MS preservation, contraction to PGF2a increased. Wrinkling increased and actin-myosin cables shortened after GM and EC; after UW, wrinkling decreased and actin-myosin cables thinned. No recovery occurred after MS. Thus the type of preservation solution influenced contractility during preservation and after rewarming. The absence of spontaneous contraction in cells cooled in UW may be advantageous.