Porous polymer scaffolds surface-modified with arginine-glycine-aspartic acid enhance bone cell attachment and differentiation in vitro
Porous polymer scaffolds surface-modified with arginine-glycine-aspartic acid enhance bone cell attachment and differentiation in vitro
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DOI：
10.1002/jbm.a.10438
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发表时间：
2003-03-01
影响因子：
4.9
通讯作者：
Hollinger, JO
中科院分区：
文献类型：
--
作者：
Hu, YH;Winn, SR;Hollinger, JO
本研究旨在确定多孔聚乳酸支架的表面修饰是否能促进成骨前体细胞(OPC)的附着、生长和分化。选择了共价接枝的氨基(-NH_2)、聚L-赖氨酸(PLL)和精氨酸-甘氨酸-天冬氨酸(RGD)作为评价指标。当时的假设是，表面修饰将对细胞-基质相互作用产生积极影响。实验通过将OPC细胞放置在聚乳酸薄膜和经NH2、PLL或RGD修饰的支架上进行组织培养。孵育24 h后检测OPC在聚乳酸薄膜上的附着情况。培养14天和28天后，分别检测碱性磷酸酶(APase)活性和钙离子水平，随着OPC向成熟骨细胞的分化，碱性磷酸酶(APase)活性和钙离子水平均升高。所有分析一式三份，数据在p小于或等于0.05时用后组正交对比(即，Fisher‘s最小显著差异)进行检验。经RGD表面修饰的聚乳酸薄膜表现出更好的OPC细胞附着性。经RGD表面修饰的聚乳酸支架上的细胞也表现出比其他支架上更高的APase活性和钙水平。这种差异在两个时间间隔都很明显，在含有成骨补充剂的组织培养液中尤其明显。本研究的结果表明，在创伤模型中，用RGD修饰聚乳酸聚合物支架表面可以增强骨细胞的附着和分化，并可能提高其再生骨组织的能力。(C)2003年威利期刊公司。
This study was designed to determine if the surface modification of porous poly(lactic acid) (PLA) scaffolds would enhance osteogenic precursor cell (OPC) attachment, growth, and differentiation. A covalently grafted amino group (-NH2), poly(L-lysine) (PLL), and the peptide arginine-glycine-aspartic acid (RGD) were selected for the evaluation. The hypothesis was that surface modification would have a positive impact,on cell-substratum interactions. The experiment was performed by OPC cells being placed on PLA films and scaffolds modified with NH2, PLL, or RGD in tissue culture media. OPC attachment to PLA films was assessed after 24 h of incubation. The growth and differentiation of the adherent OPCs on porous PLA scaffolds were assessed after 14 and 28 days for alkaline phosphatase (APase) activity and calcium levels, both of which increase as OPCs differentiate into mature bone cells. All assays were accomplished in triplicate, and data were tested with post hoc orthogonal contrasts (i.e., Fisher's least significant difference) at p less than or equal to 0.05. The PLA film surface-modified with RGD showed better OPC cell attachment than the other films. The cells on the PLA scaffolds surface-modified with RGD also exhibited an increase in APase activity and calcium levels in comparison with those on other scaffolds. This difference was apparent at both time intervals and was especially evident in the tissue culture media containing an osteogenic supplement. The results of this study indicate that modifying the surface of PLA polymer scaffolds with RGD enhances bone cell attachment and differentiation and may improve their ability to regenerate bone tissue more efficiently in wound models. (C) 2003 Wiley Periodicals, Inc.