Identification of novel steroidogenic factor 1 (SF-1)-target genes and components of the SF-1 nuclear complex
Identification of novel steroidogenic factor 1 (SF-1)-target genes and components of the SF-1 nuclear complex
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DOI：
10.1016/j.mce.2014.11.019
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发表时间：
2015-06
影响因子：
4.1
通讯作者：
T. Mizutani;Shinya Kawabe;Shin Ishikane;Yoshitaka Imamichi;A. Umezawa;K. Miyamoto
中科院分区：
文献类型：
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作者：
T. Mizutani;Shinya Kawabe;Shin Ishikane;Yoshitaka Imamichi;A. Umezawa;K. Miyamoto
类固醇生成因子 1 (SF-1) 是肾上腺和生殖发育及功能的主要调节因子。尽管 SF-1 被确定为类固醇代谢酶的转录调节因子，但研究表明 SF-1 还调节参与各种细胞过程的其他基因。之前，我们证明将 SF-1 引入间充质干细胞会导致这些细胞分化为类固醇谱系。通过使用这种分化方法，我们进行了全面分析，以确定新的 SF-1 靶基因和 SF-1 核复合物的成分。使用启动子平铺阵列和DNA微阵列进行全基因组分析，鉴定出10个基因作为新的SF-1靶基因，包括谷胱甘肽S-转移酶A家族、5-氨基乙酰丙酸合酶1和铁氧还蛋白还原酶。使用核蛋白的 SF-1 免疫亲和层析，然后进行 MS/MS 分析，我们鉴定了 24 种蛋白，包括 CCAAT/增强子结合蛋白 β，作为 SF-1 核复合物的组成部分。在这篇综述中，我们将描述新发现的类固醇生成基因的新作用。
Steroidogenic factor 1 (SF-1) is a master regulator of adrenal and reproductive development and function. Although SF-1 was identified as a transcriptional regulator for steroid metabolic enzymes, it has been shown that SF-1 also regulates other genes that are involved in various cellular processes. Previously, we showed that introduction of SF-1 into mesenchymal stem cells resulted in the differentiation of these cells to the steroidogenic lineage. By using this method of differentiation, we performed comprehensive analyses to identify the novel SF-1-target genes and components of the SF-1 nuclear complex. Genome-wide analyses with promoter tiling array and DNA microarray identified 10 genes as novel SF-1-target genes includingglutathione S-transferase Afamily,5-aminolevulinic acid synthase 1andferredoxin reductase. Using SF-1 immuno-affinity chromatography of nuclear proteins followed by MS/MS analysis, we identified 24 proteins including CCAAT/enhancer-binding protein β as components of SF-1 nuclear complex. In this review, we will describe novel roles of the newly identified genes for steroidogenesis.