Characterization of lipid composition in stimulated human lymphocytes by 1H-NMR.
Characterization of lipid composition in stimulated human lymphocytes by 1H-NMR.
复制标题
通过 1 H-NMR 表征受刺激的人淋巴细胞中的脂质成分。
DOI：
10.1016/0167-4889(90)90241-5
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Jardetzky,O
中科院分区：
文献类型：
--
作者：
Sze,DY;Jardetzky,O
最近的体内核磁共振研究引起了人们对增殖活动期间细胞脂质结构变化的兴趣。通过提取脂质和500 MHz1H-NMR测定，研究了人外周血淋巴细胞在有丝分裂刺激增殖过程中质膜脂质和细胞总脂质的变化。共振被分配使用一维和二维光谱技术，和信号独特的某些种类的脂质被确定。含胆碱和乙醇胺的脂类、甘油磷脂骨架、鞘脂、胆固醇、磷脂原和三酰基甘油容易检测。尽管使用了高分辨率技术，但仍无法对许多脂类进行分辨。增殖诱导的最容易测定的参数，即总胆固醇与总磷脂的摩尔比，磷脂酰胆碱、磷脂酰乙醇胺和鞘脂组成的核磁共振值与传统方法一致。磷脂和脂肪酸谱在质膜和总细胞脂质之间存在差异，用于静息值和对有丝分裂原的反应。
Recent in vivo NMR studies have raised interest in the structural changes of cellular lipids during proliferative activity. We investigated the changes in plasma membrane lipid and total cell lipid during mitogenically-stimulated proliferation of human peripheral blood lymphocytes by extraction of lipids and assay by 500 MHz1H-NMR. Resonances were assigned using one- and two-dimensional spectroscopic techniques, and signals unique to certain species of lipid were identified. Choline and ethanolamine-containing lipids, glycerophospholipid backbones, sphingolipids, cholesterol, plasmalogens and triacylglycerols were readily detected. Resolution of a number of lipid species was not possible, despite the use of high-resolution techniques. NMR values for proliferation-induced changes in the most easily determined parameters, namely the total cholesterol to total phospholipid molar ratio, and phosphatidylcholine, phosphatidylethanolamine and sphingolipid composition, were found to agree with traditional methods. Differences in phospholipid and fatty acid profiles were found between plasma membranes and total cell lipid for resting values and for response to mitogen.