Recruitment of Rab27a to phagosomes controls microbial antigen cross-presentation by dendritic cells.
Recruitment of Rab27a to phagosomes controls microbial antigen cross-presentation by dendritic cells.
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Rab27a 募集至吞噬体可控制树突状细胞的微生物抗原交叉呈递。
DOI：
10.1128/iai.01044-08
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发表时间：
2008
影响因子：
3.1
通讯作者：
Boes,Marianne
中科院分区：
文献类型：
--
作者：
Kim,SeongHyun;Visser,Annelies;Cruijsen,Carin;vanderVelden,AdrianusWM;Boes,Marianne
健康个体的组织和血液中存在多反应性免疫球蛋白（Ig）和补体成分。它们促进病原体的吸收和吞噬细胞溶酶体的失活，从而提供对感染的快速保护。树突状细胞（dc）是一种吞噬细胞，它可以从被吞噬的抗原中获得肽，从而引起CD8+T淋巴细胞的细胞毒性免疫反应。选择多肽进行交叉呈现的机制尚未完全解决。在这里，我们研究了多反应性Ig和补体在指导吞噬体抗原交叉呈递加工中的作用。通过血清调节促进的吞噬作用需要Ig的存在才能有效地交叉呈递微生物源性抗原。血清中补体C3的存在促进了吞噬作用，但吞噬体在抗原降解方面存在缺陷。小的GTPase Rab27a最近与抗原交叉呈递有关，并且仅在Ig存在时才迅速被招募到吞噬体中。我们的数据表明，通过多反应性Ig预结合抗原，通过募集Rab27a，增强了抗原向CD8+T细胞交叉呈递的效率。
Polyreactive immunoglobulins (Ig) and complement components are present in tissues and blood of healthy individuals. They facilitate pathogen uptake and inactivation in lysosomes of phagocytes and thereby provide rapid protection against infection. Dendritic cells (DCs) are phagocytes that can acquire peptides from phagocytosed antigen to elicit cytotoxic immune responses by CD8+T lymphocytes. The mechanisms that select peptides for cross-presentation are not fully resolved. Here we investigated the role of polyreactive Ig and complement in directing phagosomal antigen processing for cross-presentation. Phagocytosis facilitated by serum opsonization required the presence of Ig for effective antigen cross-presentation of microbe-derived antigen. The presence of complement C3 in serum promoted phagocytosis, yet phagosomes were defective in antigen degradation. The small GTPase Rab27a was recently implicated in antigen cross-presentation and was rapidly recruited to phagosomes only when Ig was present. Our data suggest that prebinding of antigen by polyreactive Ig potentiates the efficiency of antigen cross-presentation to CD8+T cells through recruitment of Rab27a.