Lovastatin enhances phenylbutyrate-induced MR-visible glycerophosphocholine but not apoptosis in DU145 prostate cells.
Lovastatin enhances phenylbutyrate-induced MR-visible glycerophosphocholine but not apoptosis in DU145 prostate cells.
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Lovastatin 增强苯丁酸诱导的 MR 可见甘油磷酸胆碱，但不增强 DU145 前列腺细胞的凋亡。
DOI：
10.1016/j.bbalip.2007.05.010
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Delikatny,EJames
中科院分区：
文献类型：
--
作者：
Milkevitch,Matthew;Jeitner,ThomasM;Beardsley,NancyJ;Delikatny,EJames
In this study the effects of lovastatin on DU145 prostate cancer cells treated with phenylbutyrate (PB) was investigated in order to determine the NMR-detectable metabolic changes resulting from the cooperative activity of these two agents. DU145 cells were perfused with PB in the presence or absence of 10 μM of the HMG-CoA reductase inhibitor lovastatin, and the results monitored by31P and diffusion-weighted1H NMR spectroscopy. Lovastatin had additive effects on the PB-induced NMR-visible total choline in1H spectra, and glycerophosphocholine in31P spectra but no significant effect on NMR-visible lipid. Moreover, lovastatin had no effect on the ability of PB to either promote the formation of oil red O-detectable lipid droplets or arrest the cell cycle. The most remarkable observations from these studies were that lovastatin enhanced the increase in glycerophosphocholine while reversing late markers of apoptosis and the loss of NTP caused by PB. These results identify a branch point separating the neutral lipid production and the apoptotic cell death caused by the actions of differentiating agents.
