Phylogenetic Characterizations of Highly Mutated EV-B106 Recombinants Showing Extensive Genetic Exchanges with Other EV-B in Xinjiang, China.
Phylogenetic Characterizations of Highly Mutated EV-B106 Recombinants Showing Extensive Genetic Exchanges with Other EV-B in Xinjiang, China.
复制标题
高度突变的 EV-B106 重组体与中国新疆其他 EV-B 存在广泛的基因交换的系统发育特征
DOI：
10.1038/srep43080
复制
发表时间：
2017-02-23
影响因子：
4.6
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Song Y;Zhang Y;Fan Q;Cui H;Yan D;Zhu S;Tang H;Sun Q;Wang D;Xu W
人肠道病毒B106(EV-B106)是B型肠病毒的新成员。到目前为止，EV-B106的核苷酸序列只有3个，GenBank数据库中只有一个全长基因组序列(云南株148/YN/CHN/12)。本研究对新疆分离的4株EV-B106毒株中国进行了系统发育分析。核苷酸序列和氨基酸序列的比对表明，新疆EV-B106株与云南EV-B106株的核苷酸同源性仅为80.5-80.8%，氨基酸同源性为95.4-97.3%，具有较强的致突变性。相似性分析和BootScan分析表明，4株新疆EV-B106毒株均在非结构区发生了频繁的型间重组。这四株毒株可能与2011年在新疆流行的EV-B85毒株具有相同的供体序列，表明这些毒株之间存在广泛的基因交流。新疆EV-B106株均对温度敏感。在新疆两个地区进行的EV-B106抗体血清阳性率研究也显示，中和抗体滴度较低，表明人群接触和传播有限。本研究将EV-B106全基因组序列送入GenBank数据库，为中国地区EV-B106的分子流行病学研究提供了有价值的信息。
Human enterovirus B106 (EV-B106) is a new member of the enterovirus B species. To date, only three nucleotide sequences of EV-B106 have been published, and only one full-length genome sequence (the Yunnan strain 148/YN/CHN/12) is available in the GenBank database. In this study, we conducted phylogenetic characterisation of four EV-B106 strains isolated in Xinjiang, China. Pairwise comparisons of the nucleotide sequences and the deduced amino acid sequences revealed that the four Xinjiang EV-B106 strains had only 80.5–80.8% nucleotide identity and 95.4–97.3% amino acid identity with the Yunnan EV-B106 strain, indicating high mutagenicity. Similarity plots and bootscanning analyses revealed that frequent intertypic recombination occurred in all four Xinjiang EV-B106 strains in the non-structural region. These four strains may share a donor sequence with the EV-B85 strain, which circulated in Xinjiang in 2011, indicating extensive genetic exchanges between these strains. All Xinjiang EV-B106 strains were temperature-sensitive. An antibody seroprevalence study against EV-B106 in two Xinjiang prefectures also showed low titres of neutralizing antibodies, suggesting limited exposure and transmission in the population. This study contributes the whole genome sequences of EV-B106 to the GenBank database and provides valuable information regarding the molecular epidemiology of EV-B106 in China.