Genetic analysis of capsular polysaccharide synthesis gene clusters in 79 capsular types of Klebsiella spp.
Genetic analysis of capsular polysaccharide synthesis gene clusters in 79 capsular types of Klebsiella spp.
复制标题
79种囊菌类型Klebsiella spp中囊膜多糖合成基因簇的遗传分析。
DOI：
10.1038/srep15573
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发表时间：
2015-10-23
影响因子：
4.6
通讯作者：
Wang JT
中科院分区：
文献类型：
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作者：
Pan YJ;Lin TL;Chen CT;Chen YY;Hsieh PF;Hsu CR;Wu MC;Wang JT
在克雷伯氏菌属中共报告了79种荚膜类型，而荚膜多糖合成（CPS）区仅在22种类型中可用。由于血清分型的局限性，完整的cps库将有助于荚膜基因分型。因此，我们解决了其余57个cps，并进行了比较分析。对来自cps位点的1,515个预测蛋白质的聚类结果将具有相似性的蛋白质分类为同源组（HGs），揭示了77个Wzy聚合酶被分类为56个HGs，这表明不同类型之间的wzy具有高度特异性。因此，基于wzy的荚膜基因分型可以区分除了那些缺乏wzy的荚膜类型（K29和K50），那些共享相同wzy的荚膜类型（K22 vs. K37）;并且应该小心地应用于那些表现出高度相似性的荚膜类型（K12 vs. K41，K2 vs. K13，K74 vs. K80，K79 vs. KN 1和K30 vs. K69）。比较CPS结构在几个荚膜类型，共享其基因内容的相似性意味着可能的功能的糖基转移酶。因此，我们的研究结果提供了各种类型克雷伯氏菌的完整cps集，其使得能够理解基因和CPS结构之间的关系，并且可用于鉴定记录的或新的荚膜类型。
A total of 79 capsular types have been reported in Klebsiella spp., whereas capsular polysaccharide synthesis (cps) regions were available in only 22 types. Due to the limitations of serotyping, complete repertoire of cps will be helpful for capsular genotyping. We therefore resolved the rest 57 cps and conducted comparative analysis. Clustering results of 1,515 predicted proteins from cps loci categorized proteins which share similarity into homology groups (HGs) revealing that 77 Wzy polymerases were classified into 56 HGs, which indicate the high specificity of wzy between different types. Accordingly, wzy-based capsular genotyping could differentiate capsule types except for those lacking wzy (K29 and K50), those sharing identical wzy (K22 vs. K37); and should be carefully applied in those exhibited high similarity (K12 vs. K41, K2 vs. K13, K74 vs. K80, K79 vs. KN1 and K30 vs. K69). Comparison of CPS structures in several capsular types that shared similarity in their gene contents implies possible functions of glycosyltransferases. Therefore, our results provide complete set of cps in various types of Klebsiella spp., which enable the understandings of relationship between genes and CPS structures and are useful for identification of documented or new capsular types.