Major epidemic clones of Staphylococcus aureus in Nigeria
Major epidemic clones of Staphylococcus aureus in Nigeria
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DOI：
10.1089/mdr.2005.11.115
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发表时间：
2005-06-01
影响因子：
2.6
通讯作者：
Van Belkum, A
中科院分区：
文献类型：
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作者：
Adesida, S;Boelens, H;Van Belkum, A
金黄色葡萄球菌临床分离株（n = 276）是在尼日利亚西南部（伊巴丹市和拉各斯市）和中北部（乔斯市）地区的 10 家不同医院收集的。这些菌株的耐药性分析表明，绝大多数菌株仍然对甲氧西林敏感（98.6% MSSA）。对这些菌株进行的脉冲场凝胶电泳 (PFGE) 鉴定出 45 种不同的基因型，其中 9 种是在四家或更多不同的医院中鉴定的。主要 PFGE 类型（基因型 2）占所有分离株的 23%。此外，其他几种菌株也被证明在个别医院中流行。通过基于阵列的多位点序列分型 (MLST) 确定，检测到的四分之三的多重耐药 MRSA 菌株为序列类型 8 (ST8)。用于对葡萄球菌盒染色体 (SCCmec) 类型进行编目的 PCR 对其中两个基因呈阴性，表明在 ST8 遗传背景中存在新的甲氧西林抗性基因复合体。总之，MSSA 主要克隆在尼日利亚流行，MRSA 发病率仍然较低。然而，在一些生态丰富的 MSSA 菌株中出现新旧版本的 SCCmec 应该被视为一个严重的警告，因为此类菌株的克隆扩张并非史无前例。
Clinical isolates of Staphylococcus aureus (n = 276) were collected in 10 different hospitals located in the Southwest (cities of Ibadan and Lagos) and North-Central (city of Jos) parts of Nigeria. Resistance profiling of these strains revealed that the vast majority was still susceptible to methicillin (98.6% MSSA). Pulsed field gel electrophoresis (PFGE) performed for these strains identified 45 different genotypes, nine of which were identified in four or more different hospitals. The major PFGE type (genotype 2) comprised 23% of all isolates. In addition, several other strains were shown to be endemic in individual hospitals. Three out of four multi-resistant MRSA strains that were detected were sequence type 8 (ST8) as determined by array-based multi-locus sequence typing (MLST). PCRs for cataloguing the staphylococcal cassette chromosome (SCCmec) type were negative for two of these, suggesting the existence of a new methicillin-resistance gene complex in the ST8 genetic background. In conclusion, major clones of MSSA circulate in Nigeria, and the MRSA incidence is still low. However, the occurrence of old and new versions of SCCmec in some of the ecologically abundant MSSA strains should be taken as a serious warning since clonal expansion of this type of strains is not unprecedented.