The effects of cryopreservation on sperm morphology, motility and mitochondrial function
The effects of cryopreservation on sperm morphology, motility and mitochondrial function
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DOI：
10.1093/humrep/17.3.704
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发表时间：
2002-03-01
影响因子：
6.1
通讯作者：
Lewis, SEM
中科院分区：
文献类型：
--
作者：
O'Connell, M;McClure, N;Lewis, SEM
背景技术背景：同时测定冷冻损伤对人精子线粒体功能、运动力、形态和活力的影响。实验方法：采用罗丹明123（R123）摄取率（精子%）和染色强度测定50名男性不育症患者精液冷冻前后精子线粒体活性。采用Tygerberg严格标准评估形态学，并采用曙红Y评估活力。采用计算机辅助精液分析（CASA）测量精子活力。结果：冷冻-解冻导致精子正常形态减少37%（P = 0.001）。所有CASA精子活力参数，除了头部横向位移幅度同样降低。精子线粒体内R123的摄取和强度分别降低了36%和47%（均P = 0.001）。此外，精子活力也有类似的显著下降（31%，P = 0.001）。结论：精子的形态、活力、线粒体活性和存活率对冷冻保存引起的损伤同样敏感。R123强度是这种创伤前后线粒体功能的一种新的和可靠的指标。
BACKGROUND: The effects of cryoinjury were determined simultaneously on the mitochondrial function, motility, morphology and viability of ejaculated human sperm. METHOD: Rhodamine 123 (R123) uptake (% of sperm) and stain intensity were used to determine sperm mitochondrial activity before and after cryopreservation from the semen of 50 men attending for infertility investigation. Morphology was assessed using Tygerberg's strict criteria and viability was assessed by eosin Y. Sperm motility was measured using computer-assisted semen analysis (CASA). RESULTS: Freeze-thawing caused a 37% (P = 0.001) reduction in normal morphological forms of sperm. All CASA sperm motility parameters except amplitude of lateral head displacement were similarly reduced. R123 uptake and intensity within sperm mitochondria decreased by 36 and 47% respectively (both P = 0.001). In addition, there was a similar significant decrease (31 %, P = 0.001) in the viability of the sperm. CONCLUSIONS: Sperm morphology, motility, mitochondrial activities and viability are equally susceptible to cryopreservation-induced damage. R123 intensity is a novel and robust indicator of mitochondrial function before and after such trauma.