Formation of proliferative tetraploid cells after treatment of diploid cells with sodium butyrate in rat 3Y1 fibroblasts
Formation of proliferative tetraploid cells after treatment of diploid cells with sodium butyrate in rat 3Y1 fibroblasts
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丁酸钠处理大鼠3Y1成纤维细胞二倍体细胞后增殖四倍体细胞的形成
DOI：
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发表时间：
1985
影响因子：
5.6
通讯作者：
G. Kimura
中科院分区：
文献类型：
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作者：
Koji Yamada;G. Kimura
当随机增殖的大鼠3Y1成纤维细胞用丁酸钠处理时，至少在G1/S边界前12小时，超过90%的细胞被可逆地阻滞，DNA含量为2C。当在早期S期同步的细胞用丁酸盐处理时，大约70%的细胞被捕获，DNA含量为4C。单一抑制剂在G1和G2相中的阻滞表明这两个相具有共同的机制。细胞按时进行有丝分裂的能力随着G2期的阻滞时间而迅速丧失。去除抑制剂后，含有4C DNA含量的细胞进入新的S期，没有介入有丝分裂。这样产生的四倍体细胞和二倍体细胞一样快速增殖。这些结果表明，正常G2导线的抑制在某种程度上负责增殖多倍体细胞的形成。
When randomly proliferating rat 3Y1 fibroblasts were treated with sodium butyrate, more than 90% of their cells were arrested reversibly with a 2C DNA content at least 12 h before the G1/S boundary. When cells synchronized in the early S phase were treated with butyrate, approximately 70% of all cells were arrested with a 4C DNA content. The arrests in both G1 and G2 phases by the single inhibitor suggest that the two phases share a common mechanism. The ability of cells to undergo mitosis on time was quickly lost with time of arrest in the G2 phase. Upon removal of the inhibitor, the cells arrested with a 4C DNA content entered a new S phase without intervening mitosis. The tetraploid cells thus produced kept proliferating as fast as diploid cells. These results suggest that the inhibition of the normal G2 traverse is somehow responsible for the formation of the proliferative polyploid cells.