Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis.
Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis.
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六种寄生原生动物的遗传特征：随机引物 DNA 分型和多位点酶电泳之间的奇偶性。
DOI：
10.1073/pnas.90.4.1335
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发表时间：
1993
影响因子：
11.1
通讯作者：
Ayala,FJ
中科院分区：
文献类型：
--
作者：
Tibayrenc,M;Neubauer,K;Barnabé,C;Guerrini,F;Skarecky,D;Ayala,FJ
我们用随机扩增多态性DNA技术对几种寄生原虫的遗传多态性进行了分析。其中一个目标是确定随机扩增多态性DNA标记是否适用于研究遗传和进化问题，特别是在不适合传统遗传分析方法的生物中，例如寄生原生动物。另一个目标是测试关于克氏锥虫和其他原虫的某些假说，这些假说是通过多位点酶电泳法建立的。随机扩增多态性DNA分析结果证实了克氏锥虫种群结构为克隆的假说，并与酶电泳法得到的克隆谱系的系统发育相一致。这两组结果的一致性证实了随机扩增多态性DNA标记是可靠的遗传标记。该标记也适用于重建物种系统发育和作为物种和亚种谱系的诊断特征。由于可以随意有效地增加引物的数量，因此，可以通过随机扩增的方法检测到的DNA多态的数量似乎几乎是无限的。随机扩增多态性DNA方法非常适合于研究某些生物体的遗传和进化问题，因为它具有成本效益，而且不需要事先了解该生物体的遗传学知识。
We have assayed genetic polymorphisms in several species of parasitic protozoa by means of random amplified polymorphic DNA (RAPD). One goal was to ascertain the suitability of RAPD markers for investigating genetic and evolutionary problems, particularly in organisms, such as the parasitic protozoa, unsuitable for traditional methods of genetic analysis. Another goal was to test certain hypotheses concerning Trypanosoma cruzi, and other protozoa, that have been established by multilocus enzyme electrophoresis. The RAPD results corroborate the hypothesis that the population structure of T. cruzi is clonal and yield a phylogeny of the clonal lineages in agreement with the one obtained by enzyme electrophoresis. This parity between the two sets of results confirms that RAPD markers are reliable genetic markers. The RAPD markers are also suitable for reconstructing species phylogenies and as diagnostic characters of species and subspecific lineages. The number of DNA polymorphisms that can be detected by the RAPD method seems virtually unlimited, since the number of primers can be increased effectively at will. The RAPD method is well suited for investigating genetic and evolutionary questions in certain organisms, because it is cost effective and demands no previous genetic knowledge about the organism.