Nutrients and the Pkh1/2 and Pkc1 Protein Kinases Control mRNA Decay and P-body Assembly in Yeast
Nutrients and the Pkh1/2 and Pkc1 Protein Kinases Control mRNA Decay and P-body Assembly in Yeast
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DOI：
10.1074/jbc.m110.196030
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发表时间：
2011-03-18
影响因子：
4.8
通讯作者：
Dickson, Robert C.
中科院分区：
文献类型：
--
作者：
Luo, Guangzuo;Costanzo, Michael;Dickson, Robert C.
受调控的mRNA衰变对真核生物的生存至关重要，但调控整体衰变并使其与生长、营养和环境线索协调的机制尚不清楚。在这里，我们表明，一个信号转导途径，包含Pkh 1/Pkh 2蛋白激酶和它们的效应激酶之一，Pkc 1，是必需的，并调节全球mRNA衰变在deadenylation步骤在酿酒酵母。此外，许多应力破坏蛋白质合成并从多聚核糖体释放mRNA，以掺入P体中进行降解或储存。我们发现，Pkh 1/2-Pkc 1途径也需要应力诱导的P体组装。Pkh-Pkc 1途径控制mRNA衰变和P体组装仅发生在营养不良的培养基中，这表明这些过程在进化中具有新的作用。我们确定的信号通路调节全球mRNA衰变和P-体组装提供了一种手段，以协调mRNA衰变与其他细胞过程的增长和长期生存所必需的。哺乳动物可能使用类似的调节机制，因为衰变装置和信号通路的组成部分是保守的。
Regulated mRNA decay is essential for eukaryotic survival but the mechanisms for regulating global decay and coordinating it with growth, nutrient, and environmental cues are not known. Here we show that a signal transduction pathway containing the Pkh1/Pkh2 protein kinases and one of their effector kinases, Pkc1, is required for and regulates global mRNA decay at the deadenylation step in Saccharomyces cerevisiae. Additionally, many stresses disrupt protein synthesis and release mRNAs from polysomes for incorporation into P-bodies for degradation or storage. We find that the Pkh1/2-Pkc1 pathway is also required for stress-induced P-body assembly. Control of mRNA decay and P-body assembly by the Pkh-Pkc1 pathway only occurs in nutrient-poor medium, suggesting a novel role for these processes in evolution. Our identification of a signaling pathway for regulating global mRNA decay and P-body assembly provides a means to coordinate mRNA decay with other cellular processes essential for growth and long-term survival. Mammals may use similar regulatory mechanisms because components of the decay apparatus and signaling pathways are conserved.