An unexpected diterpene cyclase from rice: Functional identification of a stemodene synthase
An unexpected diterpene cyclase from rice: Functional identification of a stemodene synthase
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DOI：
10.1016/j.abb.2005.09.001
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发表时间：
2006-04-15
影响因子：
3.9
通讯作者：
Peters, Reuben J.
中科院分区：
文献类型：
--
作者：
Morrone, Dana;Jin, Yinghua;Peters, Reuben J.
我们已经从水稻中克隆了一个新的二萜合酶（OsKSL 11），其从间-柯巴基二磷酸产生stemod-13（17）-烯。值得注意的是，这个基因序列没有从水稻的广泛序列信息预测。尽管进行了广泛的植物化学研究，但这种二萜或任何衍生的天然产物以前也没有在水稻植物中报道过。OsKSL 11代表了第一个被鉴定的stemodene合酶，其催化stemodane家族的二萜类天然产物的生物合成中的关键步骤，其中一些具有抗病毒活性。此外，OsKSL 11是高度同源的机械相似的stemarene合成酶最近确定的前水稻，使这对二萜环化酶的一个很好的模型系统，用于调查酶的决定因素，差异产品的结果。这种环化酶及其产物的意外性质与最近在拟南芥中观察到的前植物先前未被识别的天然产物代谢相似，这表明许多植物物种（如果不是全部的话）将被证明具有广泛的生物合成能力。(c)2005年爱思唯尔公司All rights reserved.
We have cloned a novel diterpene synthase (OsKSL11) from rice that produces stemod-13(17)-ene from syn-copalyl diphosphate. Notably, this gene sequence was not predicted from the extensive sequence information available for rice. nor, despite extensive phytochemical investigations, has this diterpene or any derived natural product previously been reported in rice plants. OsKSL11 represents the first identified stemodene synthase, which catalyzes the committed step in biosynthesis of the stemodane family of diterpenoid natural products, some of which possess antiviral activity. In addition, OsKSL11 is highly homologous to the mechanistically similar stemarene synthase recently identified front rice, making this pair of diterpene cyclases an excellent model system for investigating the enzymatic determinants for differential product outcome. The unexpected nature of this cyclase and its product parallels recent observations of preplant previously unrecognized natural products metabolism in Arabidopsis thaliana, suggesting that many, if not all, plant species will prove to have extensive biosynthetic capacity. (c) 2005 Elsevier Inc. All rights reserved.