Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen
Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen
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DOI：
10.1016/j.ijbiomac.2020.01.128
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发表时间：
2020-04-01
影响因子：
8.2
通讯作者：
Takagi, Yasuaki
中科院分区：
文献类型：
--
作者：
Meng, Dawei;Li, Wen;Takagi, Yasuaki
非哺乳动物胶原蛋白由于其作为组织工程细胞支架来源的潜力而引起了人们的极大关注。由于胶原纤维的形态控制细胞增殖和分化，因此其调节对于制造具有期望特性的支架是必不可少的。在这项研究中，我们评估了磷酸根离子（Pi）浓度的影响，形成的原纤维的特性，从鱼鳔I型胶原蛋白（SBC）和皮肤I型胶原蛋白（SC）从贝斯特鲟鱼。Pi浓度的增加降低了原纤形成速率，促进了粗原纤的形成，并增加了SBC和SC的原纤的热稳定性。然而，SBC和SC原纤表现出不同的原纤形成速率，程度的fibrillogenesis，形态，和变性温度相同的反应条件。最后，通过调节Pi浓度，可将各种类型的SBC和SC原纤维包被在细胞培养威尔斯孔上，并可在其上培养成纤维细胞。结果表明，细纤维促进成纤维细胞的延伸和增殖，而粗纤维抑制成纤维细胞的延伸，但它们在同一方向上。这项研究的结果表明，SBC纤维，表现出不同的形态，是适合用作一种新型的支架材料，其特性可以很容易地通过改变Pi浓度定制。(C)2020由Elsevier B.V.出版
Nonmammalian collagens have attracted significant attention owing to their potential for use as a source of cell scaffolds for tissue engineering. Since the morphology of collagen fibrils controls cell proliferation and differentiation, its regulation is essential for fabricating scaffolds with desirable characteristics. In this study, we evaluated the effects of the phosphate ion (Pi) concentration on the characteristics of fibrils formed from swim bladder type I collagen (SBC) and skin type I collagen (SC) from the Bester sturgeon. An increase in the Pi concentration decreased the fibril formation rate, promoted the formation of thick fibrils, and increased the thermal stability of the fibrils for both SBC and SC. However, the SBC and SC fibrils exhibited different fibril formation rates, degrees of fibrillogenesis, morphologies, and denaturation temperatures for the same reaction conditions. Finally, by regulating the Pi concentration, various types of SBC and SC fibrils could be coated on cell culture wells, and fibroblasts could be cultured on them. The results showed that thin fibrils enhance fibroblast extension and proliferation, whereas thick fibrils restrain fibroblast extension but orient them in the same direction. The results of this study suggest that SBC fibrils, which exhibit diverse morphologies, are suitable for use as a novel scaffold material, whose characteristics can be tailored readily by varying the Pi concentration. (C) 2020 Published by Elsevier B.V.