Safety and whole-body antioxidant potential of a novel anthocyanin-rich formulation of edible berries
Safety and whole-body antioxidant potential of a novel anthocyanin-rich formulation of edible berries
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DOI：
10.1007/s11010-006-1030-6
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发表时间：
2006-01-01
影响因子：
4.3
通讯作者：
Sen, CK
中科院分区：
文献类型：
--
作者：
Bagchi, D;Roy, S;Sen, CK
富含花青素的可食用浆果提取物具有广泛的治疗、药理和抗癌特性。六浆果提取物（野生蓝莓，越橘，蔓越莓，接骨木，乌藨子种子和草莓），单独和组合，在我们的实验室进行了研究的抗氧化功效，细胞毒性潜力，细胞吸收和抗血管生成特性。评价可食用浆果提取物的组合以开发协同配方OptiBerry，与测试的其他组合相比，OptiBerry表现出高氧自由基吸收能力（ORAC）值、低细胞毒性和上级抗血管生成特性。本研究旨在确定OptiBerry在体内的广谱安全性和抗氧化潜力。OptiBerry在大鼠中的急性经口LD 50大于5 g/kg。OptiBerry的急性皮肤LD 50大于2 g/kg。尸检后未观察到体重变化或不良反应。在新西兰白化病兔中进行了主要皮肤和眼刺激性研究。OptiBerry被归类为对皮肤有轻微刺激（原发性皮肤刺激指数0.3）和对眼睛有轻微刺激（最大平均总分6.0）。通过评估组织中的GSH氧化还原状态以及独特的最先进的全身氧化还原状态电子顺磁共振（EPR）成像，在大鼠和小鼠中研究了OptiBerry的抗氧化潜力。采用临床相关高压氧（HBO）暴露系统（2 atm，2 h）研究OptiBerry的抗氧化性能。OptiBerry喂养（8周）可显著预防维生素E缺乏的Sprague道利大鼠的肺和肝脏中HBO诱导的GSH氧化。此外，OptiBerry喂养的小鼠，当暴露于HBO，表现出显着的保护，在全身HBO诱导的氧化相比，未喂养的控制EPR成像。总之，这些结果表明OptiBerry是相当安全的，并具有抗氧化性能。
Edible berry extracts rich in anthocyanins possess a broad spectrum of therapeutic, pharmacologic and anti-carcinogenic properties. Six berry extracts (wild blueberry, bilberry, cranberry, elderberry, raspberry seeds and strawberry), singly and in combination, were studied in our laboratories for antioxidant efficacy, cytotoxic potential, cellular uptake and anti-angiogenic properties. Combinations of edible berry extracts were evaluated to develop a synergistic formula, OptiBerry, which exhibited high oxygen radical absorbance capacity (ORAC) value, low cytotoxicity and superior anti-angiogenic properties compared to the other combinations tested. The current study sought to determine the broad spectrum safety and antioxidant potential of OptiBerry in vivo. Acute oral LD50 of OptiBerry was greater than 5 g/kg in rats. Acute dermal LD50 of OptiBerry was greater than 2 g/kg. No changes in the body weight or adverse effects were observed following necropsy. Primary skin and eye irritation studies were conducted in New Zealand albino rabbits. OptiBerry was classified as slightly irritating to the skin (primary skin irritation index 0.3) and minimally irritating to the eye (maximum mean total score 6.0). The antioxidant potential of OptiBerry was investigated in rats and mice by assessing GSH redox status in tissues as well as by a unique state-of-the-art electron paramagnetic resonance (EPR) imaging of whole-body redox status. A clinically relevant hyperbaric oxygen (HBO) exposure system (2 atm, 2 h) was employed to study the antioxidant properties of OptiBerry. OptiBerry feeding (8 weeks) significantly prevented HBO-induced GSH oxidation in the lung and liver of vitamin E-deficient Sprague Dawley rats. Furthermore, OptiBerry-fed mice, when exposed to HBO, demonstrated significant protection in whole-body HBO-induced oxidation compared to the unfed controls by EPR imaging. Taken together, these results indicate that OptiBerry is reasonably safe and possess antioxidant properties.