Direct Mapping from Intracellular Chemotaxis Signaling to Single-Cell Swimming Behavior
Direct Mapping from Intracellular Chemotaxis Signaling to Single-Cell Swimming Behavior
复制标题
从细胞内趋化信号传导到单细胞游泳行为的直接映射
DOI：
10.1016/j.bpj.2020.10.040
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发表时间：
2020
影响因子：
3.4
通讯作者：
Yuan Junhua
中科院分区：
文献类型：
--
作者：
Tao Antai;Zhang Rongjing;Yuan Junhua
细菌的趋化性使细菌能够感知化学环境并相应地调节它们的游泳行为。虽然细胞内趋化性信号通路已被广泛研究，但仍然缺乏实验研究，可以提供从通路输出（磷酸化形式的反应调节剂磷酸化CheY（CheY-P）的细胞内浓度）到单细胞游泳行为的直接联系。在这里，我们测量了单个大肠杆菌细胞的游泳行为，同时检测细胞内的CheY-P浓度，从而提供了细胞内的CheY-P浓度和单细胞的奔跑和翻滚行为之间的直接关系。测得的关系是一致的超灵敏度的电机开关和“否决模型”，描述了个人鞭毛之间的相互作用，虽然从投票机制的贡献不能被排除。
Bacterial chemotaxis allows bacteria to sense the chemical environment and modulate their swimming behavior accordingly. Although the intracellular chemotaxis signaling pathway has been studied extensively, experimental studies are still lacking that could provide direct link from the pathway output (the intracellular concentration of the phosphorylated form of the response regulator phosphorylated CheY (CheY-P)) to single-cell swimming behavior. Here, we measured the swimming behavior of individualEscherichia colicells while simultaneously detecting the intracellular CheY-P concentration, thereby providing a direct relationship between the intracellular CheY-P concentration and the single-cell run-and-tumble behavior. The measured relationship is consistent with the ultrasensitivity of the motor switch and a "veto model" that describes the interaction among individual flagella, although contribution from the voting mechanism could not be ruled out.