Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.

Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.
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2 微米圆位点特异性重组酶的底物识别:最小底物对称元件内突变的影响。

DOI:
10.1128/mcb.6.12.4329-4334.1986
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发表时间:
1986
影响因子:
5.3
通讯作者:
Jayaram,M
Jayaram,M
中科院分区:
生物学2区
文献类型:
--
作者:
Prasad,PV;Horensky,D;Young,LJ;Jayaram,M

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2µm环状位点特异性重组酶FLP的最小底物由一个几乎完美的13碱基对二分体对称性和一个8碱基对核心组成。通过使用一系列化学合成的FLP底物在体外FLP重组和FLP结合试验,我们已经确定了四个位置内的每个对称元素是重要的接触点的FLP蛋白。此外,结合和重组数据提供了证据的两个对称元素之间的基板和两个伙伴基板的对称元素之间的FLP重组的协同性。
The minimal substrate for the 2µm circle site-specific recombinase FLP consists of a nearly perfect 13-base-pair dyad symmetry with an 8-base-pair core. By using a series of chemically synthesized FLP substrates in in vitro FLP recombination and FLP-binding assays, we have identified four positions within each of the symmetry elements that are important contact points for the FLP protein. Furthermore, the binding and recombination data provide evidence for cooperativity between the two symmetry elements of a substrate and between the symmetry elements of two partner substrates during FLP recombination.
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