Broad spectrum anti-RNA virus activities of titanium and vanadium substituted polyoxotungstates
Broad spectrum anti-RNA virus activities of titanium and vanadium substituted polyoxotungstates
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DOI：
10.1016/s0166-3542(03)00009-3
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发表时间：
2003-05-01
影响因子：
7.6
通讯作者：
Yamase, T
中科院分区：
文献类型：
--
作者：
Shigeta, S;Mori, S;Yamase, T
用钒或钛原子取代的七种多钨酸盐检测了它们对黄病毒科（登革热病毒）的活性。DFV）、正粘病毒科（A型流感病毒，fluV-A）、副粘病毒科（呼吸道合胞病毒，RSV、2型副流感病毒，PfluV-2和犬瘟热病毒，CDV）和慢病毒科（1型人免疫缺陷病毒，HIV-1）家族。在所检测的七种多钨酸盐中，PM-43 {K-5[SiVW 11 O 40]}、PM-47 {K-7[BVV 11 O 40]}和PM-1001 [K 10 Na（VO）（3）（SbW 9 O 33）（2）] 26 H（2）O含有钒。PM-1002与PM-1001具有相同的核心结构（VO）（3）（SbW 9 O33）（2），但PM-1001的三个V原子由两个V-IV和一个V-V组成，PM-1002的三个V原子由三个V-IV组成。另一方面，PM-518 {[Et 2NH 2]（7）[PTi 2 W10 O 40]}、PM-520 [Pri（2）NH（2）]（5）[PTiW 11 O 40]和PM-523 [Pri NH（3）]（6）H[PTi 2 W10 O38（O-2）（2）]H2O都含有钛。除了PM-43、PM-518和PM-523在大于或等于50 μ M时对PfluV-2不显示抑制活性外，所有化合物对所有检测的病毒均显示广谱抗病毒活性。分别为CDV和DFV。所有的化合物在小于2.0 μ M的EC 50下对HIV复制具有抑制作用。其中，PMs-1001和PMs-1002的抑制作用最强。该化合物在200 μ M的浓度下对MDCK、HEp-2和Vero细胞没有毒性。对于指数生长的MT-4细胞，含钒的多氧六酸盐（PM-43、47、1001、1002）在41和47 μ M之间的浓度下显示毒性。另一方面，含钛的聚氧乙烯酸盐（PM-518，-520，-523）在100 μ M时没有毒性。分析了PM-1001的抗HIV作用机制：影响HIV与细胞膜的结合和感染细胞与未感染细胞之间合胞体的形成。(C)2003 Elsevier Science B. V.保留所有权利。
Seven polyoxotungstates substituted with vanadium or titanium atoms were examined for their activity against Flaviviridae (Dengue fever virus. DFV), Orthomyxoviridae (influenza virus type A, fluV-A), Paramyxoviridae (respiratory syncytial virus, RSV, parainfluenza virus type 2, PfluV-2 and canine distemper virus, CDV) and Lentiviridae (human immunodeficiency virus type 1, HIV-1) families. Among the seven polyoxotungstates examined, PM-43 {K-5[SiVW11O40]}, PM-47 {K-7[BVW11O40]}, and PM-1001 [K10Na(VO)(3)(SbW9O33)(2)]26H(2)O contained vanadium. PM-1002 had the same core structure of (VO)(3)(SbW9O33)(2) as PM-1001; however, three V atoms of PM-1001 consisted of two V-IV and one V-V and those of PM-1002 consisted of three V-IV. On the other hand, PM-518 {[Et2NH2](7)[PTi2W10O40]}, PM-520 [Pri(2)NH(2)](5)[PTiW11O40] and PM-523 [PriNH(3)](6)H[PTi2W10O38(O-2)(2)]H2O all contained titanium. All compounds showed broad spectrum antiviral activity against all viruses examined except for PMs-43, -518 and -523 which did not exhibit inhibitory activity at greater than or equal to50 muM against PfluV-2. CDV and DFV, respectively. All compounds were inhibitory against HIV replication at an EC50 of less than 2.0 muM. Among them, PMs-1001 and -1002 showed the most potent inhibition. The compounds were not toxic for MDCK, HEp-2 and Vero cells at a concentration of 200 muM. For the exponentially growing MT-4 cells, the vanadium containing polyoxometalates (PMs-43, 47, 1001, 1002) showed toxicity at concentrations between 41 and 47 muM. On the other hand, titanium containing polyoxometalates (PMs-518, -520,-523) were not toxic at 100 muM. The mechanism of anti-HIV action of PM-1001 was analyzed: it affected the binding of HIV to the cell membrane and syncytium formation between HIV-infected and uninfected cells. (C) 2003 Elsevier Science B.V. All rights reserved.