HIGH-LEVELS OF INTERLEUKIN-10 IN SERUM ARE ASSOCIATED WITH FATALITY IN MENINGOCOCCAL DISEASE
HIGH-LEVELS OF INTERLEUKIN-10 IN SERUM ARE ASSOCIATED WITH FATALITY IN MENINGOCOCCAL DISEASE
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DOI：
10.1128/iai.63.6.2109-2112.1995
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发表时间：
1995-06-01
影响因子：
3.1
通讯作者：
WAAGE, A
中科院分区：
文献类型：
--
作者：
LEHMANN, AK;HALSTENSEN, A;WAAGE, A
白细胞介素10（IL-10）在体外和内毒素血症小鼠模型中抑制促炎细胞因子的产生，并已被建议作为治疗细菌性败血症的候选药物。为了研究IL-10在脑膜炎球菌病中的作用，采用夹心IL-10酶放大灵敏度免疫测定法定量测定41例脑膜炎球菌菌血症或脑膜炎伴或不伴感染性休克患者血清和脑脊液样本中的IL-10。脑膜炎球菌感染性休克患者的血清中显示出高水平的IL-10（平均值，21，221 pg/ml;范围，25至64，500 pg/ml）。所有涉及死亡的病例血清中IL-10水平均大于或等于1，000 pg/ml（平均值为23，058 pg/ml;范围为1，000至64，500 pg/ml）。无感染性休克的脑膜炎双球菌性脑膜炎患者血清中IL-10浓度极低（平均值为119 pg/ml，范围为0 - 1,050 pg/ml），但脑脊液中IL-10的释放呈区室化。血清中IL-10浓度与先前报道的相同患者血清中肿瘤坏死因子α、IL-6和IL-8浓度呈正相关。我们的结论是，在脑膜炎球菌感染性休克的初始阶段，IL-10与促炎细胞因子一起沿着被广泛激活，并且IL-10与脑膜炎球菌病的死亡率相关。
Interleukin 10 (IL-10) suppresses the production of proinflammatory cytokines in vitro and in murine models of endotoxemia and has been suggested as a candidate for treatment of bacterial septicemia. To investigate the role of IL-10 in meningococcal disease, a sandwich IL-10 enzyme-amplified sensitivity immunoassay was used to quantitate IL-10 in serum and cerebrospinal fluid samples from 41 patients with meningococcal bacteremia or meningitis with or without septic shock. High levels of IL-10 were demonstrated in sera from patients with meningococcal septic shock (mean, 21,221 pg/ml; range, 25 to 64,500 pg/ml). All cases involving fatalities had IL-10 levels in serum of greater than or equal to 1,000 pg/ml (mean, 23,058 pg/ml; range, 1,000 to 64,500 pg/ml). Patients with meningococcal meningitis without septic shock had comparably low concentrations of IL-10 in serum (mean, 119 pg/ml; range, 0 to 1,050 pg/ml) but exhibited compartmentalized release of IL-10 in cerebrospinal fluid. Concentrations of IL-10 in serum were positively correlated with the previously reported concentrations of tumor necrosis factor alpha, IL-6, and IL-8 in serum in the same patients. We conclude that IL-10 is extensively activated along with the proinflammatory cytokines during the initial phase of meningococcal septic shock and that IL-10 is associated with fatality in meningococcal disease.