Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)
Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)
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DOI：
10.1007/s11032-015-0318-1
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发表时间：
2015-05
影响因子：
3.1
通讯作者：
Wenxing Pang;Xiaonan Li;S. Choi;Van Dan Nguyen;Vignesh Dhandapani;Yoon-young Kim;N. Ramchiary;
中科院分区：
文献类型：
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作者：
Wenxing Pang;Xiaonan Li;S. Choi;Van Dan Nguyen;Vignesh Dhandapani;Yoon-young Kim;N. Ramchiary;
白菜裂头会严重影响白菜的品质和商品价值。以抗劈头自交系“748”与易劈头自交系“747”杂交得到的f2群体为研究对象，构建了劈头抗性遗传图谱。该图谱全长830.9 cM，包含270个标记，分布在9个连锁群中，分别对应b的9条染色体。oleracea。相邻标记之间的平均距离为3.6 cM。在第2、4和6号染色体上共检测到6个抗头裂的数量性状位点。在2年的实验中，分别在2号染色体和4号染色体上检测到两个qtl，分别为spl -2-1和spl -4-1，表明这两个潜在位点在控制抗劈头性状中起重要作用。在保守的qtlspll -2-1区域检测到与劈头抗性紧密相关的标记BRPGM0676和BRMS137。同源性分析表明，sl -2-1锚定在b的一个3.18 mb的基因组区域。拟南芥1号染色体核型E区与十字花科植物祖先同源。此外，利用场发射扫描电子显微镜观察到两个亲本系在细胞结构方面存在显著差异。与抗性品系“748”相比，“747”细胞壁更薄，细胞密度更低，细胞尺寸更大，细胞壁结构异常。不同的细胞结构可为评价白菜裂头提供细胞学依据。
Cabbage head splitting can greatly affect both the quality and commercial value of cabbage (Brassica oleracea). To detect the genetic basis of head-splitting resistance, a genetic map was constructed using an F2population derived by crossing “748” (head-splitting-resistant inbred line) and “747” (head-splitting-susceptible inbred line). The map spans 830.9 cM and comprises 270 markers distributed in nine linkage groups, which correspond to the nine chromosomes ofB. oleracea. The average distance between adjacent markers was 3.6 cM. A total of six quantitative trait loci (QTLs) conferring resistance to head splitting were detected in chromosome 2, 4, and 6. Two QTLs,SPL-2-1andSPL-4-1, on chromosomes 2 and 4, respectively, were detected in the experiments over 2 years, suggesting that these two potential loci were important for governing the head-splitting resistance trait. Markers BRPGM0676 and BRMS137, which were tightly linked with head-splitting resistance, were detected in the conserved QTLSPL-2-1region using bulked segregant analysis. Synteny analysis showed thatSPL-2-1was anchored to a 3.18-Mb genomic region of theB. oleraceagenome, homologous to crucifer ancestral karyotype E block in chromosome 1 ofArabidopsis thaliana.Moreover, using a field emission scanning electron microscope, significant differences were observed between the two parental lines in terms of cell structures. Line “747” had thinner cell wall, lower cell density, larger cell size, and anomalous cell wall structure compared with the resistant line “748.” The different cell structures can provide a cytological base for assessing cabbage head splitting.