Influence of humoral regulators on proliferation and maturation of normal and leukemic cells.
Influence of humoral regulators on proliferation and maturation of normal and leukemic cells.
复制标题
体液调节剂对正常和白血病细胞增殖和成熟的影响。
作者：
J. Karp;P. Burke
在血清中可以检测到影响骨髓细胞增殖的因素。为了确定细胞的功能并比较这些因素的影响，我们从正常人和白血病患者的骨髓中获得了细胞。观察不同时间(0~6d)药物诱导的刺激和抑制血清以及白血病前血清对正常和白血病骨髓细胞增殖和粒细胞形态的影响。刺激血清促进细胞增殖，而抑制血清和白血病预处理血清抑制正常细胞和恶性细胞的增殖。将正常增殖细胞暴露于抑制性或白血病预处理血清中，成熟粒细胞的数量增加最多。相反，尽管白血病细胞对这些血清的增殖反应与正常相似，但成熟程度很低。这些数据表明，白血病前处理血清类似于抑制血清，不具有致白血病作用。两者都抑制正常和白血病骨髓细胞的增殖，同时促进正常细胞的成熟。然而，白血病成髓细胞不能通过这些体液调节因子而成熟。
Factors that influence the proliferation of marrow elements can be detected in sera. To determine the function and to compare the effect of these factors, cells were obtained from patients with normal and leukemic bone marrows. The effects of drug-induced stimulatory and inhibitory sera and leukemic pretreatment sera over time (0 to 6 days) on proliferation and granulocytic morphology of normal and leukemic bone marrow cells in culture were evaluated. Increased proliferation was associated with stimulatory sera, while inhibitory and leukemic pretreatment sera retarded proliferation of both normal and malignant cells. Exposure of normal proliferative cells to inhibitory or leukemic pretreatment sera yielded the greatest increase in mature granulocyte forms. In contrast, although the proliferative response of leukemic cells to these sera was similar to normal, maturation was minimal. These data suggest that leukemic pretreatment sera are similar to inhibitory sera and are not leukemogenic. Both retard proliferation of normal and leukemic bone marrow cells while enhancing maturation of normal cells. Leukemic myeloblasts, however, cannot be made to mature by these humoral regulators.