Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
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多个基因编码人类 Na ,K -ATP 酶催化亚基。
DOI：
10.1073/pnas.84.12.4039
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发表时间：
1987
影响因子：
11.1
通讯作者：
Lingrel,JB
中科院分区：
文献类型：
--
作者：
Shull,MM;Lingrel,JB
构建了人基因组文库，并用绵羊和大鼠分别编码Na+，K+-ATPase催化亚基的α和α(+)亚型的杂交探针进行了筛选。分离得到了跨越150kb的基因组序列。通过限制性内切酶图谱、Southern杂交分析和限制性DNA测序鉴定了4个基因，分别命名为αA、αB、αC和αD，每个基因的长度为20-25kb。我们提出的证据表明，其中两个基因，阿尔法A和阿尔法B，分别编码阿尔法和阿尔法(+)亚型。另外两个基因，αC和αD，其中一个与α(+)基因有物理上的连锁，它们的核苷酸和氨基酸与Na+，K+-ATPase催化亚单位的cDNA序列有同源性，但并不对应于任何已发现的亚型。
A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.