INFLUENZA VIRUS-A INFECTION INDUCES INTERLEUKIN-8 GENE-EXPRESSION IN HUMAN AIRWAY EPITHELIAL-CELLS
INFLUENZA VIRUS-A INFECTION INDUCES INTERLEUKIN-8 GENE-EXPRESSION IN HUMAN AIRWAY EPITHELIAL-CELLS
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DOI：
10.1016/0014-5793(92)80799-m
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发表时间：
1992-09-14
期刊：
影响因子：
3.5
通讯作者：
JACOBY, DB
中科院分区：
文献类型：
--
作者：
CHOI, AMK;JACOBY, DB
为了确定呼吸道上皮细胞在介导病毒诱导的炎症中的作用，我们用人类A型流感病毒/Port Chalmers/72(H3N2)感染原代培养的人呼吸道上皮细胞。两天后收集上清液，采用酶联免疫吸附试验检测趋化细胞因子IL-8的水平。提取细胞RNA，Northern印迹分析IL-8mRNA的表达。病毒感染后白介素8的产量增加了一倍多，而白介素8的信使核糖核酸增加了四倍。因此，诱导病毒感染的呼吸道上皮细胞中IL-8基因表达可能是导致病毒诱导的呼吸道炎症的重要早期步骤。
To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemotactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-fold. Thus induction of interleukin-8 gene expression in virus-infected airway epithelium may be an important early step leading to virus-induced airway inflammation.