Controlled recruitment of monocytes and macrophages to specific organs through transgenic expression of monocyte chemoattractant protein-1.
Controlled recruitment of monocytes and macrophages to specific organs through transgenic expression of monocyte chemoattractant protein-1.
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DOI：
10.4049/jimmunol.155.12.5769
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发表时间：
1995-12
影响因子：
4.4
通讯作者：
M. Fuentes;S. Durham;M. Swerdel;A. Lewin;D. Barton;J. Megill;R. Bravo;S. Lira
中科院分区：
文献类型：
--
作者：
M. Fuentes;S. Durham;M. Swerdel;A. Lewin;D. Barton;J. Megill;R. Bravo;S. Lira
在胸腺和中枢神经系统中过度表达趋化因子单核细胞趋化蛋白-1 （MCP-1）的转基因小鼠在这些组织中的单核细胞数量高于对照组。在胸腺中，Mac-1和F4/80阳性细胞数量略有增加，但淋巴样细胞数量无明显变化。在大脑中表达MCP-1的转基因小鼠中检测到更明显的单核浸润。根据光镜、超微结构和免疫组织化学标准，大脑中绝大多数募集的细胞是单核细胞和巨噬细胞。免疫组织化学显示，这类细胞位于血管周围方向，很少有实质浸润，可能是MCP-1在血管中积累的结果。LPS处理可显著增强脑内单核细胞浸润，提示MCP-1的募集特性可通过其他因素增强。
Transgenic mice overexpressing the chemokine monocyte chemoattractant protein-1 (MCP-1) in the thymus and central nervous system have a higher number of mononuclear cells in those tissues than do control littermates. In the thymus, there is a modest increase in the number of Mac-1 and F4/80 positive cells, but no apparent change in the number of lymphoid cells. A more pronounced mononuclear infiltrate is detected in transgenic mice expressing MCP-1 in the brain. The vast majority of the recruited cells in the brain are monocytes and macrophages, as defined by light microscopy, and ultrastructural and immunohistochemical criteria. Such cells are found in a perivascular orientation with minimal parenchymal infiltration, possibly as a consequence of the accumulation of MCP-1 in the vessels, as shown by immunohistochemistry. The mononuclear cell infiltrate in the brain can be significantly amplified by LPS treatment, suggesting that the recruitment properties of MCP-1 can be potentiated by additional factors.