Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II.
Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II.
复制标题
DOI：
10.1093/jb/mvm047
复制
发表时间：
2007-03
影响因子：
2.7
通讯作者：
Zenta Yasukawa;C. Sato;K. Kitajima
中科院分区：
文献类型：
--
作者：
Zenta Yasukawa;C. Sato;K. Kitajima
急性期蛋白是炎症的重要标志，有时在组织损伤的一般防御反应中起作用。在本研究中，我们鉴定了一个32kda的蛋白，该蛋白与单克隆抗体2-4B （mAb）具有免疫反应性。2-4B)，它对di/oligoNeu5Gc结构具有特异性，并且在松节油或脂多糖刺激后表现为急性期蛋白。基于基质辅助激光解吸/电离飞行时间质谱分析，鉴定该32kda蛋白为碳酸酐酶II （CA-II）。小鼠和人CA-II具有免疫反应性，用单抗免疫沉淀。2-4B，但不含唾液酸。除了mAb。2-4B，单克隆抗体。S2-566是一种针对含有dineu5ac的聚糖的抗体，能够识别CA-II，而抗低聚/聚唾液酸抗体则不能。这些结果表明CA-II蛋白结构的一部分模拟单克隆抗体识别的二二酸结构。这是首次报道CA-II在炎症后的血清中循环。
Acute-phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32-kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute-phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32-kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.