Characterization of phosphodiesterase 1 in human malignant melanoma cell lines.
Characterization of phosphodiesterase 1 in human malignant melanoma cell lines.
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发表时间：
2009-04
影响因子：
2
通讯作者：
K. Shimizu;T. Murata;Yoshihiro Watanabe;C. Sato;Hiroshi Morita;T. Tagawa
中科院分区：
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作者：
K. Shimizu;T. Murata;Yoshihiro Watanabe;C. Sato;Hiroshi Morita;T. Tagawa
来自盘基网柄藻的分化诱导因子1 [DIF-1; 1-（3，5-二氯-2，6-二羟基-4-甲氧基苯基）己-1-酮]在哺乳动物细胞中表现出抗增殖活性。我们以前已经表明，磷酸二酯酶1（PDE 1）是DIF-1的药理学靶点，但没有关于PDE 1在人恶性黑色素瘤细胞中的报道。因此，我们的特点是PDE 1在人恶性黑色素瘤MAA细胞。材料和方法研究MAA细胞中PDE 1 mRNA的表达。克隆人PDE 1C 1和PDE 1C 3的完全开放阅读框。通过MTS [3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑鎓，内盐]试验测定细胞生长。结果在MAA细胞中检测到PDE 1C mRNA的表达。PDE 1C 1的核苷酸序列与先前发表的人PDE 1C 1的核苷酸序列相同。在PDE 1C 3的核苷酸2246处，A被G取代，但这并不改变编码的氨基酸。PDE 1抑制剂vinpocetin可抑制细胞生长。结论PDE 1C mRNA在人恶性黑色素瘤MAA细胞中有表达，并可能起重要作用。
BACKGROUND Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl) hexan-1-one] from Dictyostelium discoideum exhibits antiproliferative activity in mammalian cells. We have previously shown that phosphodiesterase 1 (PDE1) is a pharmacological target of DIF-1, but there are no reports of PDE1 in human malignant melanoma cells. Therefore, we characterized PDE1 in human malignant melanoma MAA cells. MATERIALS AND METHODS PDE1 mRNA expression was investigated in MAA cells. The full open reading frames for human PDE1C1 and PDE1C3 were cloned. Cell growth was determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assay. RESULTS PDE1C mRNA expression was detected in MAA cells. The nucleotide sequence of PDE1C1 was identical to that of human PDE1C1, previously published. At nucleotide 2246 in PDE1C3, A was replaced by G, but this did not change the encoded amino acid. Cell growth was inhibited by the PDE1 inhibitor vinpocetin. CONCLUSION PDE1C mRNA is expressed and may play an important role in human malignant melanoma MAA cells.