Stable in-vivo electrochemical sensing of tonic serotonin levels using PEDOT/CNT-coated glassy carbon flexible microelectrode arrays.
Stable in-vivo electrochemical sensing of tonic serotonin levels using PEDOT/CNT-coated glassy carbon flexible microelectrode arrays.
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DOI：
10.1016/j.bios.2023.115242
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发表时间：
2023-03-27
影响因子：
12.6
通讯作者：
Cui，Xinyan Tracy
中科院分区：
文献类型：
--
作者：
Castagnola，Elisa;Robbins，Elaine M.;Cui，Xinyan Tracy
脑内5-羟色胺（5-HT）浓度的慢性采样对于跟踪神经系统疾病的发展和药物治疗的时间过程至关重要。尽管它们的价值，在体内慢性多部位测量强直5-HT尚未报道。为了填补这一技术空白，我们批量制造可植入的玻璃碳（GC）微电极阵列（MEA）到一个灵活的SU-8基板，以提供一个电化学稳定和生物相容性的设备/组织接口。为了实现对5-HT浓度的检测，我们应用了聚（3，4-乙撑二氧噻吩）/碳纳米管（PEDOT/CNT）修饰的GC微电极对5-HT具有很高的灵敏度，良好的抗污染性，对常见的神经化学干扰物具有很好的选择性。我们的PEDOT/CNT包被的GCMEA成功地检测了麻醉和清醒小鼠海马CA 2区域内不同位置的基础5-HT浓度。此外，PEDOT/CNT-涂层MEA能够检测到紧张性5-HT在小鼠海马体植入后一周。组织学显示，与市售的刚性硅探针相比，柔性GC MEA植入物在海马体中引起较少的组织损伤和减少的炎症反应。据我们所知，这种PEDOT/CNT涂层的GC MEA是第一个可植入的，灵活的传感器，能够慢性生活多网站感测的紧张性5-HT。
Chronic sampling of tonic serotonin (5-hydroxytryptamine, 5-HT) concentrations in the brain is critical for tracking neurological disease development and the time course of pharmacological treatments. Despite their value,in vivochronic multi-site measurements of tonic 5-HT have not been reported. To fill this technological gap, we batch-fabricated implantable glassy carbon (GC) microelectrode arrays (MEAs) onto a flexible SU-8 substrate to provide an electrochemically stable and biocompatible device/tissue interface. To achieve detection of tonic 5-HT concentrations, we applied a poly(3,4-ethylenedioxythiophene)/carbon nanotube (PEDOT/CNT) electrode coating and optimized a square wave voltammetry (SWV) waveform for selective 5-HT measurement.In vitro, the PEDOT/CNT-coated GC microelectrodes achieved high sensitivity to 5-HT, good fouling resistance, and excellent selectivity against the most common neurochemical interferents.In vivo, our PEDOT/CNT-coated GC MEAs successfully detected basal 5-HT concentrations at different locations within the CA2 region of the hippocampus of both anesthetized and awake mice. Furthermore, the PEDOT/CNT-coated MEAs were able to detect tonic 5-HT in the mouse hippocampus for one week after implantation. Histology reveals that the flexible GC MEA implants caused less tissue damage and reduced inflammatory response in the hippocampus compared to commercially available stiff silicon probes. To the best of our knowledge, this PEDOT/CNT-coated GC MEA is the first implantable, flexible sensor capable of chronicin vivomulti-site sensing of tonic 5-HT.