Determination of leucocyte subsets in human saliva by flow cytometry
Determination of leucocyte subsets in human saliva by flow cytometry
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DOI：
10.1016/j.archoralbio.2011.10.015
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发表时间：
2012-05-01
影响因子：
3
通讯作者：
Sabioncello, Ante
中科院分区：
文献类型：
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作者：
Vidovic, Andelko;Juras, Danica Vidovic;Sabioncello, Ante
目的：验证一种基于流式细胞仪检测石蜡刺激的人全唾液中主要白细胞亚群[中性粒细胞、单核细胞、T细胞和B细胞]的方法的有效性。设计：用四色流式细胞仪检测8名健康志愿者唾液中的白细胞亚群，连续3天。比较唾液和全血中的白细胞亚群。结果：唾液中PMN细胞为优势细胞。单个核细胞的比例在0.3%~7.2%之间，其中单核细胞比例最高，其次是T细胞和B细胞。不考虑个体内较高的日间变异性，白细胞亚群的比例在三次测量中没有显著变化，T细胞和单核细胞的高ICC被计算出来。结论：流式细胞术可以作为一种无创性和重复性的方法来分析人唾液中的白细胞亚群。有必要对可能影响唾液白细胞亚群的病理和其他情况进行进一步研究。(C)2011爱思唯尔有限公司。保留所有权利。
Objective: Validation of a flow cytometry-based method for the determination of major leucocyte subsets [polymorphonuclear (PMN) cells, monocytes, T cells and B cells] in paraffin-stimulated whole human saliva.Design: Salivary leucocyte subsets were determined by four-colour flow cytometry in eight healthy volunteers on three consecutive days. Comparison of leucocyte subsets between saliva and whole blood was also performed. Day-to-day variability and intraclass correlation coefficients (ICC) were determined as indicators of assay reliability.Results: It was observed that PMN cells were the predominant cells in the saliva. Percentages of mononuclear cells ranged from 0.3% to 7.2%, with monocytes composing the highest percentage, followed by T cells and B cells. Regardless of high intra-individual day-to-day variability, proportions of leucocyte subsets did not significantly change over three measurements, and high ICCs were calculated for T cells and monocytes.Conclusion: Flow cytometry can be used as non-invasive and reproducible method for the analysis of leucocyte subsets in human saliva. Further investigation of pathological and other conditions that have the potential to influence salivary leucocyte subsets is warranted. (C) 2011 Elsevier Ltd. All rights reserved.