Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging
Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging
复制标题
DOI：
10.1038/nbt1220
复制
发表时间：
2006-07-01
影响因子：
46.9
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Zhang, Hao F.;Maslov, Konstantin;Wang, Lihong V.
虽然光吸收与生物组织的生理状态密切相关，但现有的高分辨率光学成像手段，包括共焦显微镜(1，2)、双光子显微镜(3，4)和光学相干层析成像(5)，不能直接感知光吸收。此外，光学散射阻止了这些方法成像的深度超过组织表面下的1 mm。在这里，我们报告了功能光声显微镜(FPAM)，它提供光吸收的多波长成像，并允许在最大成像深度与深度分辨率之比大于100的情况下，超过这一深度限制的高空间分辨率。采用反射模式，而不是正交或透射式，因为它适用于更多的解剖部位。FPAM是通过体内血管生成、黑色素瘤、动物单个血管的血红蛋白氧饱和度(SO2)和人类总血红蛋白浓度的成像来证实的。
Although optical absorption is strongly associated with the physiological status of biological tissue, existing high-resolution optical imaging modalities, including confocal microscopy(1,2), two-photon microscopy(3,4) and optical coherence tomography(5), do not sense optical absorption directly. Furthermore, optical scattering prevents these methods from imaging deeper than similar to 1 mm below the tissue surface. Here we report functional photoacoustic microscopy (fPAM), which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100. Reflection mode, rather than orthogonal or transmission mode, is adopted because it is applicable to more anatomical sites than the others. fPAM is demonstrated with in vivo imaging of angiogenesis, melanoma, hemoglobin oxygen saturation (sO(2)) of single vessels in animals and total hemoglobin concentration in humans.