Altered regulation of aquaporin gene expression in allergen and IL-13-induced mouse models of asthma.
Altered regulation of aquaporin gene expression in allergen and IL-13-induced mouse models of asthma.
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DOI：
10.1016/j.cyto.2008.12.018
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发表时间：
2009-04
期刊：
影响因子：
3.8
通讯作者：
Ho, Melisa W. Y.
中科院分区：
文献类型：
--
作者：
Krane, Carissa M.;Deng, Bijia;Mutyam, Venkateshwar;McDonald, Casey A.;Pazdziorko, Stephen;Mason, Lawrence;Goldman, Samuel;Kasaian, Marion;Chaudhary, Divya;Williams, Cara;Ho, Melisa W. Y.
已知IL-13影响许多有助于哮喘表型的过程，包括炎症、纤维化和粘液产生。跨膜水通道的水通道蛋白（AQP）家族成员是肺损伤和炎症模型中的调节靶点。因此，我们检测了过敏原和IL-13诱导的哮喘小鼠模型中AQP mRNA和蛋白的表达。来自卵清蛋白致敏和卵清蛋白激发（OVA/OVA）和IL-13处理的小鼠的肺显示气道增厚、粘液产生增加和肺嗜酸性粒细胞增多。肺功能测试显示，与对照组相比，OVA/OVA和IL-13处理的小鼠中乙酰甲胆碱诱导的气道高反应性显著增加。定量PCR分析显示，这两种模型中的水通道蛋白的差异调节。AQP 1和AQP 4 mRNA表达在OVA/OVA模型中下调，但在IL-13模型中不下调。AQP 5 mRNA在两种模型中均降低，而AQP 3仅在IL-13模型中上调。Western分析表明，减少表达的顶部定位的水通道蛋白，（AQP 5），并伴随上调的基底外侧水通道蛋白（AQP 3或AQP 4）是两个诱导型哮喘模型的特征。这些结果表明，水通道蛋白是过敏原和IL-13诱导的哮喘小鼠模型中基因表达的共同靶标。
IL-13 is known to affect many processes that contribute to an asthmatic phenotype, including inflammation, fibrosis, and mucus production. Members of the aquaporin (AQP) family of transmembrane water channels are targets of regulation in models of lung injury and inflammation. Therefore, we examined AQP mRNA and protein expression in allergen and IL-13-induced mouse models of asthma. Lungs from ovalbumin sensitized and ovalbumin challenged (OVA/OVA) and IL-13 treated mice showed airway thickening, increased mucus production, and pulmonary eosinophilia. Pulmonary function tests showed a significant increase in methacholine-induced airway hyperreactivity in OVA/OVA and IL-13-treated mice as compared with controls. Quantitative PCR analysis revealed differential regulation of AQPs in these two models. AQP1 and AQP4 mRNA expression was downregulated in the OVA/OVA model, but not in the IL-13 model. AQP5 mRNA was reduced in both models, whereas AQP3 was upregulated only in the IL-13 model. Western analysis showed that diminished expression of an apically localized aquaporin, (AQP5), and concomitant upregulation of a basolateral aquaporin (AQP3 or AQP4) are characteristic features of both inducible asthma models. These results demonstrate that aquaporins are common targets of gene expression in both allergen and IL-13 induced mouse models of asthma.
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影响因子：
15.9
作者：
Bai, CX;Fukuda, N;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
4.5
作者：
Sato, K;Kobayashi, K;Tamai, S
通讯作者：
Tamai, S
DOI：
10.1152/ajplung.00311.2001
发表时间：
2002-02-01
影响因子：
4.9
作者：
Danahay, H;Atherton, H;Poll, CT
通讯作者：
Poll, CT
DOI：
10.1085/jgp.117.6.573
发表时间：
2001-06
期刊：
The Journal of general physiology
影响因子：
--
作者：
Song Y;Jayaraman S;Yang B;Matthay MA;Verkman AS
通讯作者：
Verkman AS
DOI：
10.1016/j.bbrc.2006.01.103
发表时间：
2006-04-14
影响因子：
3.1
作者：
Chen, ZH;Wang, XD;Bai, CX
通讯作者：
Bai, CX