INVITRO SYNTHESIS OF A PUTATIVE PRECURSOR OF SERINE-PYRUVATE AMINOTRANSFERASE OF RAT-LIVER MITOCHONDRIA
INVITRO SYNTHESIS OF A PUTATIVE PRECURSOR OF SERINE-PYRUVATE AMINOTRANSFERASE OF RAT-LIVER MITOCHONDRIA
复制标题
DOI：
10.1016/0006-291x(81)91557-6
复制
发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
TATIBANA, M
中科院分区：
文献类型：
--
作者：
ODA, T;ICHIYAMA, A;TATIBANA, M
丝氨酸：大鼠肝脏丙酮酸氨基转移酶是由线粒体中的高血糖素诱导的一种酶，是由核酸酶处理的兔网织红细胞裂解物和小麦胚芽提取物作为前体在无细胞蛋白质合成系统中合成的，推测为apprx。2000道尔顿；成熟酶的亚基。编码该前体的可翻译mRNA的肝脏水平为.apprx。在宰杀前3.5小时注射高血糖素的大鼠比对照动物高出40倍。
Serine:pyruvate aminotransferase of rat liver, an enzyme induced by glucagon in mitochondria, was synthesized in cell-free protein synthesizing systems derived from nuclease-treated rabbit reticulocyte lysate and wheat germ extract as a putative precursor which was .apprx. 2000 daltons > the subunit of mature enzyme. The hepatic level of translatable mRNA coding for the putative precursor was .apprx. 40 times higher in rats that received a glucagon administration 3.5 h before sacrifice than in control animals.