A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P.
A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P.
复制标题
大肠杆菌 tRNAtyrsu3 中的 G43 到 U43 突变影响 RNase P 的加工。
DOI：
10.1093/nar/11.5.1491
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发表时间：
1983
影响因子：
14.9
通讯作者：
Altman,S
中科院分区：
文献类型：
--
作者：
Furdon,PJ;Guerrier-Takada,C;Altman,S
大肠杆菌 tRNA1Tyrsu3+（G43 至 U43）反密码子干中的一个新突变已得到表征。噬菌体 Ø80DHA61.3（Ø80psu3+su0 的衍生物）携带的突变 tRNA 基因编码，与 Ø80psu3 相比，仅产生成熟抑制性 tRNA 的 20%。突变 tRNA 前体和成熟 tRNA 的构象都发生了改变。 Ø80DHA61.3 编码的前体 tRA 被 RNase P 处理的速度比 suØ3 慢。前体，并且不像su3前体那样形成稳定的酶-底物复合物。 Ø80DHA61.3 还包含一个大基因，该基因始于 su 基因和 su3+ 之间的 spcer 区域。基因，延伸至su0。基因，包括 tRNA 基因之后的大部分重复区域。
A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+(G43 to U43) has been characterized. The gene c⊕ding for the mutant tRNA, carried by phage Ø80DHA61.3 a derivative of Ø80psu3+su0, produces only 20% of mature suppressor tRNA as compared with Ø80psu3. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRA coded for by Ø80DHA61.3 is processed by RNase P more slowly than the suØ3. prcursor and does not form as stable an enzyme-substrate complex as does su3, precursor. Ø80DHA61.3 also +contains a largeeletion which begins in the spcer region between the su, gene and the su3+. gene, extends through the su0. gene and includes most of the repeated region following the tRNA genes.