Comparative metabolomics analysis of different resistant rice varieties in response to the brown planthopper Nilaparvata lugens Hemiptera: Delphacidae
Comparative metabolomics analysis of different resistant rice varieties in response to the brown planthopper Nilaparvata lugens Hemiptera: Delphacidae
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DOI：
10.1007/s11306-019-1523-4
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发表时间：
2019-04-01
期刊：
影响因子：
3.6
通讯作者：
Zhang, Wenqing
中科院分区：
文献类型：
--
作者：
Kang, Kui;Yue, Lei;Zhang, Wenqing
褐飞虱（Nilaparvata lugens Stal，BPH）是重要的粮食作物水稻（Oryza sativa L.）的主要害虫之一。目前，已经培育和推广了多种抗褐飞虱水稻品种来控制褐飞虱。然而，在这方面，目的采用无偏代谢组学方法研究不同水稻品种对褐飞虱的防御代谢反应及其作用方式的差异方法采用气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）技术进行代谢组学检测。研究了TN 1、IR 36和IR 56水稻品种在褐飞虱取食后不同时间点（0 h、24 h、48 h和96 h）鉴定代谢物的含量变化。结果与TN 1相比，IR 36和IR 56中大部分初级代谢产物的含量变化较为稳定，但部分防御相关代谢产物的浓度变化更为剧烈和持久。差异表达途径分析显示，在感染过程中，IR 36持续诱导氰基氨基酸和脂类代谢，而IR 56中硫胺素、牛磺酸和亚牛磺酸代谢的变化更为显著。此外，对褐飞虱适合度有害的槲皮素和亚精胺含量在TN 1和IR 36中显著升高，结论不同水稻品种对褐飞虱侵染响应的代谢产物谱的差异有助于阐明水稻在褐飞虱侵染过程中的代谢机制，为水稻品种间的代谢研究提供新的理论依据。来控制这种害虫。
Introduction The brown planthopper (BPH, Nilaparvata lugens Stal, Hemiptera: Delphacidae) is one of the most devastating insect pests of the crucially important cereal crop, rice (Oryza sativa L.). Currently, multiple BPH-resistant rice varieties have been cultivated and generalized to control BPH. However, the defence metabolic responses and their modes of action against BPH in different rice cultivars remain uncharacterized.Objective We used a non-biased metabolomics approach to explore the differences in metabolite profiles in response to BPH infestation in the susceptible TN1 rice cultivar and two resistant cultivars (IR36 and IR56).Methods The metabolomic detection based on gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) was performed to investigate the content changes of identified metabolites in TN1, IR36 and IR56 rice varieties at various time points (0 h, 24 h, 48 h and 96 h) post BPH feeding. The differentially expressed metabolites were screened and the corresponding metabolic pathways were further enriched.Results The results showed that compared to that in TN1, the content changes of most primary metabolites were more stable, but the concentration alterations of some defence-related metabolites were more acute and persistent in IR36 and IR56. Furthermore, the differentially expressed pathways analysis revealed that cyanoamino acids and lipids metabolism was persistently induced in IR36, but changes in thiamine, taurine and hypotaurine metabolism were more significant in IR56 during BPH infestation. Besides, the contents of quercetin and spermidine which were harmful to BPH fitness, were significantly elevated by BPH in TN1 and IR36, and the quercetin level was significantly decreased during BPH feeding in IR56.Conclusion The results of the differences in metabolite profiles in response to BPH infestation in different rice cultivars were useful to clarify the metabolic mechanism of rice plants during BPH infestation and to provide new resources to control this insect pest.