Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica.
Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica.
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家蝇幼虫肽组分的免疫佐剂作用
DOI：
10.1186/s12906-015-0951-6
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发表时间：
2015-12-02
影响因子：
--
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Chen L;Zhang J;Sun H
研究背景：家蝇幼虫（双翅目：家蝇科）在中医学上用于治疗气滞、褥疮、骨髓炎、脓疮、唇疱疹等，也用于治疗昏迷、胃癌等。还报告了其在未处理小鼠中与传统用途有关的免疫调节作用。然而，这种昆虫的免疫佐剂潜力尚未研究。通过检测鸡卵清蛋白（OVA）和禽流感疫苗（rL-H5）免疫小鼠后抗原特异性抗体滴度、脾细胞增殖、自然杀伤（NK）细胞活性和脾细胞分泌细胞因子的变化，评价了MDPF对鸡卵清蛋白（OVA）和禽流感疫苗（rL-H5）免疫应答的佐剂效应。MDPF不仅能显著促进刀豆球蛋白A（ConA）、脂多糖（LPS）和抗原刺激的脾细胞增殖，而且能显著提高血清抗原特异性IgG、IgG 1、IgG 2a、用OVA和rL-H5免疫的小鼠中的IgG 2b抗体滴度。MDPF对rL-H5免疫小鼠脾细胞NK细胞杀伤活性也有明显的促进作用。结论：MDPF具有增强细胞免疫和体液免疫应答的作用，并能诱导Th 1/Th 2平衡应答，是一种安全有效的疫苗佐剂候选物。
Background:The larvae of Musca domestica (Diptera: Muscidae) have been used traditionally for malnutritional stagnation, decubital necrosis, osteomyelitis, ecthyma and lip scald and also to treat coma and gastric cancer in the traditional Chinese medicine. Its immunomodulatory effects in naïve mice in relation to the traditional uses were also reported. However, the immunological adjuvant potentials of this insect have not yet been studied.Methods:The peptide fraction from the larvae of Musca domestica L. (MDPF) was evaluated for its adjuvant potentials on the immune responses to ovalbumin (OVA) and avian influenza vaccine (rL-H5) by determining antigen-specific antibody titers, splenocyte proliferation, activity of natural killer (NK) cell, the secretion of cytokines from splenocytes in the immunized mice.Results:MDPF significantly enhanced not only the concanavalin A (Con A)-, lipopolysaccharide (LPS)- and antigen-stimulated splenocyte proliferation, but serum antigen-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in the mice immunized with OVA and rL-H5. MDPF also remarkably promoted the killing activities of NK cells in splenocytes from the mice immunized with rL-H5. Furthermore, MDPF significantly promoted the production of Th1 (IL-2 and IFN-γ) and Th2 (IL-10) cytokines from splenocytes in the immunized mice.Conclusions:The results indicated that MDPF had a potential to increase both cellular and humoral immune responses and elicit a balanced Th1/Th2 response, and that MDPF may be a safe and efficacious vaccine adjuvant candidate.