The Drosophila melanogaster seminal fluid protein Acp62F is a protease inhibitor that is toxic upon ectopic expression.
The Drosophila melanogaster seminal fluid protein Acp62F is a protease inhibitor that is toxic upon ectopic expression.
复制标题
DOI：
--
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
O. Lung;U. Tram;C. Finnerty;Marcie A. Eipper-Mains;J. Kalb;M. Wolfner
中科院分区：
文献类型：
--
作者：
O. Lung;U. Tram;C. Finnerty;Marcie A. Eipper-Mains;J. Kalb;M. Wolfner
黑腹果蝇精液蛋白质刺激交配雌性的精子储存和产卵，但也导致其寿命缩短。我们在这里报告，8果蝇精液蛋白（Acps）和一个非Acp测试，只有Acp62F是有毒的异位表达时。对未成年雄性或雌性果蝇的毒性发生在一次接触后，而对成年雌性果蝇的毒性则需要多次接触。在交配期间雌性接受的Acp62F中，约10%进入循环系统，而约90%保留在生殖道中。我们发现，在生殖道，Acp62F定位于子宫腔和女性的精子储存器官。Acp62F的序列分析和生化测定表明，它编码的胰蛋白酶抑制剂的序列和结构相似的细胞外丝氨酸蛋白酶抑制剂从线虫蛔虫。鉴于先前的结果表明进入交配的女性的血淋巴中的Acp62F，我们建议，Acp62F是一个候选分子，有助于ACP依赖性降低女性寿命。我们建议，Acp62F的蛋白酶抑制剂活性发挥积极的保护功能，在交配女性的生殖道，但进入少量的这种蛋白质进入女性的血淋巴可能有助于交配的成本。
Drosophila melanogaster seminal fluid proteins stimulate sperm storage and egg laying in the mated female but also cause a reduction in her life span. We report here that of eight Drosophila seminal fluid proteins (Acps) and one non-Acp tested, only Acp62F is toxic when ectopically expressed. Toxicity to preadult male or female Drosophila occurs upon one exposure, whereas multiple exposures are needed for toxicity to adult female flies. Of the Acp62F received by females during mating, approximately 10% enters the circulatory system while approximately 90% remains in the reproductive tract. We show that in the reproductive tract, Acp62F localizes to the lumen of the uterus and the female's sperm storage organs. Analysis of Acp62F's sequence, and biochemical assays, reveals that it encodes a trypsin inhibitor with sequence and structural similarities to extracellular serine protease inhibitors from the nematode Ascaris. In light of previous results demonstrating entry of Acp62F into the mated female's hemolymph, we propose that Acp62F is a candidate for a molecule to contribute to the Acp-dependent decrease in female life span. We propose that Acp62F's protease inhibitor activity exerts positive protective functions in the mated female's reproductive tract but that entry of a small amount of this protein into the female's hemolymph could contribute to the cost of mating.